Anti-AMF antibody - N-terminal (ab226212)
Key features and details
- Rabbit polyclonal to AMF - N-terminal
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-AMF antibody - N-terminal
See all AMF primary antibodies -
Description
Rabbit polyclonal to AMF - N-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanIP HumanWB Human
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Immunogen
Synthetic peptide within Human AMF aa 1-50 (N terminal). The exact sequence is proprietary. NP_000166.2
Database link: P06744 -
Positive control
- IHC-P: Human lung carcinoma tissue. IP: HeLa whole cell lysate. WB: HeLa, HEK-293T and Jurkat whole cell lysate.
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General notes
This product was previously labelled as Glucose 6 phosphate isomerase
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-AMF antibody - N-terminal (ab226212)All lanes : Anti-AMF antibody - N-terminal (ab226212) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Developed using the ECL technique.
Exposure time: 10 seconds
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMF antibody - N-terminal (ab226212)Formalin fixed, paraffin embedded human lung carcinoma tissue stained for AFM with ab226212 (1/1000 dilution) in immunohistochemical analysis. DAB detection. Counterstain: hematoxylin (blue).
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Immunoprecipitation - Anti-AMF antibody - N-terminal (ab226212)AFM was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226212 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226212 at 1 µg/ml.
Lane 1: ab226212 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 seconds.
