Anti-Aldolase B antibody [5E2AD2] (ab129728)
![Anti-Aldolase B antibody [5E2AD2] (ab129728)](https://www.abcam.com/ps/products/129/ab129728/Images/ALDOB-Primary-antibodies-ab129728-4.jpg "Anti-Aldolase B antibody [5E2AD2] (ab129728)")
Key features and details
- Mouse monoclonal [5E2AD2] to Aldolase B
- Suitable for: IP, ICC/IF, Flow Cyt
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-Aldolase B antibody [5E2AD2]
See all Aldolase B primary antibodies -
Description
Mouse monoclonal [5E2AD2] to Aldolase B -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIP MouseRatHuman
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Immunogen
Tissue, cells or virus. This information is considered to be commercially sensitive.
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Positive control
- Human, Rat and Mouse liver homogenate lysates; HepG2 cells; Hela cells
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General notes
This antibody clone is manufactured by Abcam.
Product was previously marketed under the MitoSciences sub-brand.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99% HEPES buffered saline -
Concentration information loading... -
Purification notes
Purity is near homogeneity as judged by SDS-PAGE. ab129728 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
Clonality
Monoclonal -
Clone number
5E2AD2 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Immunoprecipitation - Anti-Aldolase B antibody [5E2AD2] (ab129728)Immunoprecipitation image of ab129728. In vertebrates, three forms of this ubiquitous glycolytic enzyme are found, aldolase A in muscle, aldolase B in liver and aldolase C in brain. Antibody ab129728 immunocaptured 39.4 kDa ALDOB only from rat liver homogenate (RLH), not from rat brain homogenate (RBH) nor rat skeletal muscle homogenate (RSMH). Gel stained with Coomassie brilliant blue G. Lane1: ladder Lane2: rat brain homogenate lysate Lane3: rat liver homogenate lysate Lane4: rat skeletal muscle homogenate lysate
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![Immunoprecipitation - Anti-Aldolase B antibody [5E2AD2] (ab129728)](https://www.abcam.com/ps/products/129/ab129728/Images/ALDOB-Primary-antibodies-ab129728-1.jpg)
Immunoprecipitation - Anti-Aldolase B antibody [5E2AD2] (ab129728)Immunoprecipitation image of ab129728. ALDOB antibody immunocaptured 39.4 kDa ALDOB from human liver homogenate (HLH), Rat liver homogenate (RLH) and Mouse liver homogenate (MLH). Gel stained with Coomassie brilliant blue G.
Lane 1: Ladder Lane 2: Human liver homogenate lysate
Lane 3: Rat liver homogenate lysate Lane 4: Mouse liver homogenate lysate
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![Immunocytochemistry/ Immunofluorescence - Anti-Aldolase B antibody [5E2AD2] (ab129728)](https://www.abcam.com/ps/products/129/ab129728/Images/ALDOB-Primary-antibodies-ab129728-2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Aldolase B antibody [5E2AD2] (ab129728)Immunocytochemistry image of ab129728 stained HepG2 cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min). The cells were incubated with ab129728 at 5 µg/ml for 2h at room temperature or over night at 4°C. The secondary antibody was (red) AlexaFluor® 594 goat anti-mouse IgG (H+L) used at 1/1000 dilution for 1h. 1% BSA was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Antigen retrieval step is recommended for a better signal. The target protein locates to the cytoplasm. -
![Flow Cytometry - Anti-Aldolase B antibody [5E2AD2] (ab129728)](https://www.abcam.com/ps/products/129/ab129728/Images/ALDOB-Primary-antibodies-ab129728-3.jpg)
Flow Cytometry - Anti-Aldolase B antibody [5E2AD2] (ab129728)Flow cytometry using ab129728. Hela cells were stained with 5 µg/mL ab129728 antibody (blue) or no primary antibody control (red) and analyzed by flow cytometry.
