Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Aldolase B + Aldolase C antibody [EPR3138Y] - BSA and Azide free (ab223527)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3138Y] to Aldolase B + Aldolase C - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Aldolase B + Aldolase C antibody [EPR3138Y] - BSA and Azide free

  • Description

    Rabbit monoclonal [EPR3138Y] to Aldolase B + Aldolase C - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC: Human, mouse and rat kidney tissues. WB: Human, mouse and rat kidney lysate. His-tagged human Aldolase B and C full-length recombinant protein.

  • General notes

    Ab223527 is the carrier-free version of ab75751. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab223527 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] - BSA and Azide free (ab223527)
    Western blot - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] - BSA and Azide free (ab223527)
    All lanes : Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (ab75751) at 1/200 dilution

    Lane 1 : His-tagged human Aldolase A full-length recombinant protein
    Lane 2 : His-tagged human Aldolase B full-length recombinant protein
    Lane 3 : His-tagged human Aldolase C full-length recombinant protein

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 39 kDa



    This data was developed using ab75751, the same antibody clone in a different buffer formulation. 

  • Western blot - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] - BSA and Azide free (ab223527)
    Western blot - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] - BSA and Azide free (ab223527)
    All lanes : Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (ab75751) at 1/1000 dilution (Purified)

    Lane 1 : Human kidney lysate
    Lane 2 : Mouse kidney lysate
    Lane 3 : Rat kidney lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 39 kDa
    Observed band size: 39 kDa



    This data was developed using ab75751, the same antibody clone in a different buffer formulation. 

    Blocking Buffer and concentration: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] - BSA and Azide free (ab223527)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] - BSA and Azide free (ab223527)

    This data was developed using ab75751, the same antibody clone in a different buffer formulation.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labeling Aldolase B with Purified ab223527 at 1:100 dilution (1.47 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] - BSA and Azide free (ab223527)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] - BSA and Azide free (ab223527)

    This data was developed using ab75751, the same antibody clone in a different buffer formulation.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling Aldolase B with Purified ab223527 at 1:100 dilution (1.47 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] - BSA and Azide free (ab223527)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] - BSA and Azide free (ab223527)

    This data was developed using ab75751, the same antibody clone in a different buffer formulation.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling Aldolase B with Purified ab223527 at 1:100 dilution (1.47 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Anti-Aldolase B + Aldolase C antibody [EPR3138Y] - BSA and Azide free (ab223527)
    Anti-Aldolase B + Aldolase C antibody [EPR3138Y] - BSA and Azide free (ab223527)

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