Anti-Thrombospondin antibody (ab88529)
Key features and details
- Mouse polyclonal to Thrombospondin
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Thrombospondin antibody
See all Thrombospondin primary antibodies -
Description
Mouse polyclonal to Thrombospondin -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Full length Human Thrombospondin protein (NP_003237.2)
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Positive control
- WB: Human pancreas tissue lysate and Thrombospondin transfected 293T cell lysate IF: HeLa cell
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4
Constituent: 2.68% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Thrombospondin antibody (ab88529)All lanes : Anti-Thrombospondin antibody (ab88529) at 1 µg/ml
Lane 1 : Thrombospondin transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 129 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-Thrombospondin antibody (ab88529)ab88529 at 10 µg/ml staining Thrombospondin in paraformaldehyde fixed, permeabilised HeLa cells, by immunofluorescence. -
Western blot - Anti-Thrombospondin antibody (ab88529)Anti-Thrombospondin antibody (ab88529) at 1 µg/ml + Human pancreas tissue lysate at 50 µg
Predicted band size: 129 kDa
Observed band size: ~ 150 kDa why is the actual band size different from the predicted?
