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Neuroscience Cell Type Marker Glia marker Astrocyte marker

Anti-Aldolase B+Aldolase C antibody [EPR3137] - BSA and Azide free (ab248483)

Price and availability

526 012 ₸

Availability

Order now and get it on Tuesday March 09, 2021

Anti-Aldolase B+Aldolase C antibody [EPR3137] - BSA and Azide free (ab248483)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3137] to Aldolase B+Aldolase C - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-Aldolase B+Aldolase C antibody [EPR3137] - BSA and Azide free
    See all Aldolase B+Aldolase C primary antibodies
  • Description

    Rabbit monoclonal [EPR3137] to Aldolase B+Aldolase C - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab248483 is the carrier-free version of ab133333 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab248483 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as ALDOB+Aldolase C

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR3137
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Glia marker
    • Astrocyte marker
    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of carbohydrates
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Carbohydrate metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia

Images

  • Western blot - Anti-Aldolase B+Aldolase C antibody [EPR3137] - BSA and Azide free (ab248483)
    Western blot - Anti-Aldolase B+Aldolase C antibody [EPR3137] - BSA and Azide free (ab248483)
    All lanes : Anti-Aldolase B+Aldolase C antibody [EPR3137] (ab133333) at 1/1000 dilution

    Lane 1 : Human Aldolase A full-length recombinant protein with His-Tag
    Lane 2 : Human Aldolase B full-length recombinant protein with His-Tag
    Lane 3 : Human Aldolase C full-length recombinant protein with His-Tag

    Lysates/proteins at 0.015 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 39 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 second


    This data was developed using ab133333, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B+Aldolase C antibody [EPR3137] - BSA and Azide free (ab248483)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B+Aldolase C antibody [EPR3137] - BSA and Azide free (ab248483)

    This data was developed using ab133333, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human fetal liver tissue staining Aldolase B + Aldolase C with ab133333 at 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B+Aldolase C antibody [EPR3137] - BSA and Azide free (ab248483)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B+Aldolase C antibody [EPR3137] - BSA and Azide free (ab248483)
    This data was developed using ab133333, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human adult liver tissue staining Aldolase B + Aldolase C with ab133333 at 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Western blot - Anti-Aldolase B+Aldolase C antibody [EPR3137] - BSA and Azide free (ab248483)
    Western blot - Anti-Aldolase B+Aldolase C antibody [EPR3137] - BSA and Azide free (ab248483)
    All lanes : Anti-Aldolase B+Aldolase C antibody [EPR3137] (ab133333) at 1/1000 dilution

    Lane 1 : Human fetal liver tissue lysate
    Lane 2 : Human fetal kidney tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP conjugated at 1/2000 dilution

    Predicted band size: 39 kDa
    Observed band size: 40 kDa why is the actual band size different from the predicted?



    This data was developed using ab133333, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B+Aldolase C antibody [EPR3137] - BSA and Azide free (ab248483)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B+Aldolase C antibody [EPR3137] - BSA and Azide free (ab248483)
    This data was developed using ab133333, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue using ab133333 showing -ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B+Aldolase C antibody [EPR3137] - BSA and Azide free (ab248483)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B+Aldolase C antibody [EPR3137] - BSA and Azide free (ab248483)
    This data was developed using ab133333, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded normal Human breast tissue using ab133333 showing -ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B+Aldolase C antibody [EPR3137] - BSA and Azide free (ab248483)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B+Aldolase C antibody [EPR3137] - BSA and Azide free (ab248483)
    This data was developed using ab133333, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded normal Human kidney tissue using ab133333 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Anti-Aldolase B+Aldolase C antibody [EPR3137] - BSA and Azide free (ab248483)
    Anti-Aldolase B+Aldolase C antibody [EPR3137] - BSA and Azide free (ab248483)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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