Anti-Aldolase B+Aldolase C antibody [EPR3137] - BSA and Azide free (ab248483)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3137] to Aldolase B+Aldolase C - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Aldolase B+Aldolase C antibody [EPR3137] - BSA and Azide free
See all Aldolase B+Aldolase C primary antibodies -
Description
Rabbit monoclonal [EPR3137] to Aldolase B+Aldolase C - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab248483 is the carrier-free version of ab133333 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab248483 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as ALDOB+Aldolase C
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR3137 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Aldolase B+Aldolase C antibody [EPR3137] (ab133333) at 1/1000 dilution
Lane 1 : Human Aldolase A full-length recombinant protein with His-Tag
Lane 2 : Human Aldolase B full-length recombinant protein with His-Tag
Lane 3 : Human Aldolase C full-length recombinant protein with His-Tag
Lysates/proteins at 0.015 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 39 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondThis data was developed using ab133333, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B+Aldolase C antibody [EPR3137] - BSA and Azide free (ab248483)
This data was developed using ab133333, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human fetal liver tissue staining Aldolase B + Aldolase C with ab133333 at 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B+Aldolase C antibody [EPR3137] - BSA and Azide free (ab248483)This data was developed using ab133333, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human adult liver tissue staining Aldolase B + Aldolase C with ab133333 at 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-Aldolase B+Aldolase C antibody [EPR3137] (ab133333) at 1/1000 dilution
Lane 1 : Human fetal liver tissue lysate
Lane 2 : Human fetal kidney tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated at 1/2000 dilution
Predicted band size: 39 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?This data was developed using ab133333, the same antibody clone in a different buffer formulation.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B+Aldolase C antibody [EPR3137] - BSA and Azide free (ab248483)This data was developed using ab133333, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue using ab133333 showing -ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B+Aldolase C antibody [EPR3137] - BSA and Azide free (ab248483)This data was developed using ab133333, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded normal Human breast tissue using ab133333 showing -ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B+Aldolase C antibody [EPR3137] - BSA and Azide free (ab248483)This data was developed using ab133333, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded normal Human kidney tissue using ab133333 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -