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Anti-Involucrin antibody [EPR13060(N)] - BSA and Azide free (ab203134)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13060(N)] to Involucrin - BSA and Azide free
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-Involucrin antibody [EPR13060(N)] - BSA and Azide free
    See all Involucrin primary antibodies

  • Description

    Rabbit monoclonal [EPR13060(N)] to Involucrin - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal skin and HT-1080 cell lysates; HACAT cells.

  • General notes

    Ab203134 is the carrier-free version of ab184969. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab203134 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Involucrin antibody [EPR13060(N)] - BSA and Azide free (ab203134)
    Western blot - Anti-Involucrin antibody [EPR13060(N)] - BSA and Azide free (ab203134)
    Anti-Involucrin antibody [EPR13060(N)] - C-terminal (ab184969) at 1/10000 dilution + Human fetal skin tissue lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 68 kDa



    This data was developed using ab184969, the same antibody clone in a different buffer formulation.

  • Flow Cytometry - Anti-Involucrin antibody [EPR13060(N)] - BSA and Azide free (ab203134)
    Flow Cytometry - Anti-Involucrin antibody [EPR13060(N)] - BSA and Azide free (ab203134)
    This data was developed using ab184969, the same antibody clone in a different buffer formulation.Flow cytometric analysis of Involucrin expression in paraformaldehyde-fixed HACAT cells using ab184969 at 1/40 dilution (red) and a rabbit IgG as negative control (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
  • Western blot - Anti-Involucrin antibody [EPR13060(N)] - BSA and Azide free (ab203134)
    Western blot - Anti-Involucrin antibody [EPR13060(N)] - BSA and Azide free (ab203134)
    Anti-Involucrin antibody [EPR13060(N)] - C-terminal (ab184969) at 1/50000 dilution + HT-1080 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 68 kDa



    This data was developed using ab184969, the same antibody clone in a different buffer formulation.

     

  • Anti-Involucrin antibody [EPR13060(N)] - BSA and Azide free (ab203134)
    Anti-Involucrin antibody [EPR13060(N)] - BSA and Azide free (ab203134)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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