Anti-Nectin-4 antibody (ab235897)
Key features and details
- Rabbit polyclonal to Nectin-4
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Nectin-4 antibody
See all Nectin-4 primary antibodies -
Description
Rabbit polyclonal to Nectin-4 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow
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Immunogen
Recombinant fragment corresponding to Human Nectin-4 aa 91-199.
Database link: Q96NY8 -
Positive control
- WB: Raji and NIH/3T3 whole cell lysate. IHC-P: Human pancreatic cancer tissue. ICC/IF: MCF7 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Nectin-4 antibody (ab235897)All lanes : Anti-Nectin-4 antibody (ab235897) at 1/500 dilution
Lane 1 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nectin-4 antibody (ab235897)Paraffin-embedded human pancreatic cancer tissue stained for Nectin-4 using ab235897 at 1/400 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at room temperature. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using a HRP conjugated SP system.
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Immunocytochemistry/ Immunofluorescence - Anti-Nectin-4 antibody (ab235897)4% formaldehyde-fixed, 0.2% Triton X-100 permeabilized MCF7 (human breast adenocarcinoma cell line) cells stained for Nectin-4 using ab235897 at 1/133 dilution in ICC/IF. The cells were blocked in 10% normal goat serum and incubated with primary antibody at 4°C overnight. Secondary antibody was Aleza Fluor® 488-conjugated goat anti-rabbit IgG (H+L).
