Anti-Nectin-4 antibody [EPR15613-68] (ab192033)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15613-68] to Nectin-4
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-Nectin-4 antibody [EPR15613-68]
See all Nectin-4 primary antibodies -
Description
Rabbit monoclonal [EPR15613-68] to Nectin-4 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- T47D, MCF7 and Human placenta lysates; Human placenta tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15613-68 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Nectin-4 antibody [EPR15613-68] (ab192033) at 1/1000 dilution
Lane 1 : T47D lysate
Lane 2 : MCF7 lysate
Lane 3 : Human placenta lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 55 kDa
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Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling Nectin-4 with ab192033 at 1/3000 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.
Inset: Negative control: using PBS instead of primary antibody.Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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