Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-KGF antibody [EPR7261] (ab131162)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7261] to KGF
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-KGF antibody [EPR7261]
    See all KGF primary antibodies

  • Description

    Rabbit monoclonal [EPR7261] to KGF

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human KGF aa 1-100. The exact sequence is proprietary.
    Database link: P21781

  • Positive control

    • WB: Fetal brain and fetal kidney lysates; 293T cell lysate. Human, mouse and rat brain lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-KGF antibody [EPR7261] (ab131162)
    Western blot - Anti-KGF antibody [EPR7261] (ab131162)
    All lanes : Anti-KGF antibody [EPR7261] (ab131162) at 0.1 µg/ml (purified)

    Lane 1 : Mouse brain lysates
    Lane 2 : Rat brain lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 23 kDa
    Observed band size: 28 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer: 5% NFDM/TBST.

  • Western blot - Anti-KGF antibody [EPR7261] (ab131162)
    Western blot - Anti-KGF antibody [EPR7261] (ab131162)
    Anti-KGF antibody [EPR7261] (ab131162) at 0.1 µg/ml (purified) + Human brain lysates at 15 µg

    Secondary
    Rabbit monoclonal [EPR7261] to KGF (ab131162) at 1/2000 dilution (Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG)

    Predicted band size: 23 kDa



    Blocking and diluting buffer: 5% NFDM/TBST.

  • Western blot - Anti-KGF antibody [EPR7261] (ab131162)
    Western blot - Anti-KGF antibody [EPR7261] (ab131162)
    All lanes : Anti-KGF antibody [EPR7261] (ab131162) at 1/1000 dilution (Unpurified)

    Lane 1 : Fetal brain lysate
    Lane 2 : 293T cell lysate
    Lane 3 : Fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 23 kDa
    Observed band size: 28 kDa why is the actual band size different from the predicted?

  • Anti-KGF antibody [EPR7261] (ab131162)
    Anti-KGF antibody [EPR7261] (ab131162)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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