Anti-Superoxide Dismutase 1 antibody (ab183881)
Key features and details
- Rabbit polyclonal to Superoxide Dismutase 1
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Superoxide Dismutase 1 antibody
See all Superoxide Dismutase 1 primary antibodies -
Description
Rabbit polyclonal to Superoxide Dismutase 1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Sheep, Cow, Dog, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Xenopus tropicalis -
Immunogen
Recombinant fragment within Human Superoxide Dismutase 1 aa 75-154. The exact sequence is proprietary.
Database link: P00441 -
Positive control
- NT2D1 , PC-3 , U87-MG , SK-N-SH , HeLa , Rat brain lysates, Hela cells, colon carcinoma, muscle, rat brain tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Superoxide Dismutase 1 antibody (ab183881) at 1/500 dilution
Lane 1 : NT2D1 whole cell lysate
Lane 2 : PC-3 whole cell lysate
Lane 3 : U87-MG whole cell lysate
Lane 4 : SK-N-SH whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 16 kDa15% SDS-PAGE
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Immunocytochemical analysis of methanol fixed Hela cells labeling Superoxide Dismutase 1 at 1/100 dilution with ab183881. Bottom panel shows co-staining with Hoechst 33342 (blue)
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Anti-Superoxide Dismutase 1 antibody (ab183881) at 1/1000 dilution + Rat brain whole cell lysate at 50 µg
Predicted band size: 16 kDa15% SDS-PAGE
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Immunohistochemical analysis of paraffin-embedded rat brain labeling Superoxide Dismutase 1 with ab183881 at 1/500 dilution
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Immunohistochemical analysis of paraffin-embedded mouse Muscle labeling Superoxide Dismutase 1 with ab183881 at 1/500 dilution
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Immunohistochemical staining of paraffin-embedded human colon carcinoma labeling Superoxide Dismutase 1 at 1/500 dilution with ab183881
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All lanes : Anti-Superoxide Dismutase 1 antibody (ab183881) at 1/500 dilution
Lane 1 : Hela mock control
Lane 2 : HeLa transfected shSuperoxide Dismutase 1
Lysates/proteins at 30 µg per lane.
Predicted band size: 16 kDa15% SDS-PAGE