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Signal Transduction Protein Trafficking Chaperones Heat Shock Proteins

Anti-Superoxide Dismutase 1 antibody [EPR1726] (ab79390)

Price and availability

328 339 ₸

Availability

Order now and get it on Wednesday March 17, 2021

Anti-Superoxide Dismutase 1 antibody [EPR1726] (ab79390)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1726] to Superoxide Dismutase 1
  • Suitable for: WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-Superoxide Dismutase 1 antibody [EPR1726]
    See all Superoxide Dismutase 1 primary antibodies
  • Description

    Rabbit monoclonal [EPR1726] to Superoxide Dismutase 1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human Superoxide Dismutase 1 aa 50-150. The exact sequence is proprietary.
    Database link: P00441
    (Peptide available as ab195608)

  • Positive control

    • WB: HeLa, MCF7, Jurkat and HT29 cell lysates
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR1726
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Chaperones
    • Heat Shock Proteins
    • Cell Biology
    • Other Antibodies
    • Oxidative Stress
    • Cancer
    • Cancer Metabolism
    • Cellular metabolic process
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Oxidative stress
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Antioxidants
    • Metabolism
    • Types of disease
    • Cancer

Images

  • Western blot - Anti-Superoxide Dismutase 1 antibody [EPR1726] (ab79390)
    Western blot - Anti-Superoxide Dismutase 1 antibody [EPR1726] (ab79390)
    All lanes : Anti-Superoxide Dismutase 1 antibody [EPR1726] (ab79390) at 1/10000 dilution (purified)

    Lane 1 : HeLa cell lysate
    Lane 2 : MCF7 cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : HT-29 cell lysate
    Lane 5 : T47-D cell lysate
    Lane 6 : A431 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

    Predicted band size: 16 kDa
    Observed band size: 18 kDa
    why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-Superoxide Dismutase 1 antibody [EPR1726] (ab79390)
    Western blot - Anti-Superoxide Dismutase 1 antibody [EPR1726] (ab79390)
    All lanes : Anti-Superoxide Dismutase 1 antibody [EPR1726] (ab79390) at 1/200000 dilution (unpurified)

    Lane 1 : HeLa cell lysate
    Lane 2 : MCF7 cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : HT29 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 16 kDa
    Observed band size: 16 kDa

  • Anti-Superoxide Dismutase 1 antibody [EPR1726] (ab79390)
    Anti-Superoxide Dismutase 1 antibody [EPR1726] (ab79390)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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