Anti-Superoxide Dismutase 1 antibody [EPR1726] (ab79390)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1726] to Superoxide Dismutase 1
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-Superoxide Dismutase 1 antibody [EPR1726]
See all Superoxide Dismutase 1 primary antibodies -
Description
Rabbit monoclonal [EPR1726] to Superoxide Dismutase 1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
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Positive control
- WB: HeLa, MCF7, Jurkat and HT29 cell lysates
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1726 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Superoxide Dismutase 1 antibody [EPR1726] (ab79390) at 1/10000 dilution (purified)
Lane 1 : HeLa cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HT-29 cell lysate
Lane 5 : T47-D cell lysate
Lane 6 : A431 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution
Predicted band size: 16 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
All lanes : Anti-Superoxide Dismutase 1 antibody [EPR1726] (ab79390) at 1/200000 dilution (unpurified)
Lane 1 : HeLa cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HT29 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 16 kDa
Observed band size: 16 kDa
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