Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Pescadillo antibody [8E9] - BSA and Azide free (ab255843)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [8E9] to Pescadillo - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Pescadillo antibody [8E9] - BSA and Azide free
    See all Pescadillo primary antibodies

  • Description

    Rat monoclonal [8E9] to Pescadillo - BSA and Azide free

  • Host species

    Rat

  • Tested Applications & Species

    Application Species
    IF
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HEK-293, HCT116, Jurkat and RAW264.7 whole cell lysate. ICC/IF: HeLa and HEK-293 cells.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab255843 is the carrier-free version of ab252849. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunofluorescence - Anti-Pescadillo antibody [8E9] - BSA and Azide free (ab255843)
    Immunofluorescence - Anti-Pescadillo antibody [8E9] - BSA and Azide free (ab255843)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed,0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) labeling Pescadillo with ab252849 at 0.978µg/ml, followed by Goat Anti-Mouse IgG (Alexa Fluor® 488) (ab150113) secondary antibody at 1/1000 dilution (green). Confocal image showing nucleolar staining on HeLa cell line. The nuclear counterstain is DAPI (blue).

    Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 594) (ab150080) secondary antibody at 1/1000 dilution (red).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252849).

  • Immunofluorescence - Anti-Pescadillo antibody [8E9] - BSA and Azide free (ab255843)
    Immunofluorescence - Anti-Pescadillo antibody [8E9] - BSA and Azide free (ab255843)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed,0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) labeling Pescadillo with ab252849 at 0.978µg/ml, followed by Goat Anti-Mouse IgG (Alexa Fluor® 488) (ab150113) secondary antibody at 1/1000 dilution (green). Confocal image showing nucleolar staining on HeLa cell line. The nuclear counterstain is DAPI (blue).

    Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 594) (ab150080) secondary antibody at 1/1000 dilution (red).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252849).

  • Western blot - Anti-Pescadillo antibody [8E9] - BSA and Azide free (ab255843)
    Western blot - Anti-Pescadillo antibody [8E9] - BSA and Azide free (ab255843)
    All lanes : Anti-Pescadillo antibody [8E9] (ab252849) at 0.489 µg/ml

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : HEK-293 (human embryonic kidney epithelial cell), whole cell lysate
    Lane 3 : HCT116 (human colorectal carcinoma epithelial cell), whole cell lysate
    Lane 4 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
    Lane 5 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/100000 dilution

    Predicted band size: 68 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1-4: 1 sec; Lane 5: 5 secs.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252849).

  • Anti-Pescadillo antibody [8E9] - BSA and Azide free (ab255843)
    Anti-Pescadillo antibody [8E9] - BSA and Azide free (ab255843)

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