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Signal Transduction Protein Trafficking Chaperones Heat Shock Proteins

Anti-Hsp70 antibody [C9] (ab239496)

Anti-Hsp70 antibody [C9] (ab239496)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [C9] to Hsp70
  • Suitable for: WB, IHC-P
  • Reacts with: Human, Pig
  • Isotype: IgG2b

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Overview

  • Product name

    Anti-Hsp70 antibody [C9]
    See all Hsp70 primary antibodies
  • Description

    Mouse monoclonal [C9] to Hsp70
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    Pig
    See all applications and species data
  • Immunogen

    Recombinant fragment (His-T7-tag) corresponding to Human Hsp70 aa 250 to the C-terminus. Two N-terminal tags, His-tag and T7-tag (Expressed in E.coli).
    Database link: P0DMV9

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • IHC-P: Human liver cancer tissue. WB: Pig liver and brain lysates; HeLa and Jurkat cell lysates; Recombinant human Hsp70 protein.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    Purified from TCS.
  • Clonality

    Monoclonal
  • Clone number

    C9
  • Isotype

    IgG2b
  • Light chain type

    kappa
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Chaperones
    • Heat Shock Proteins
    • Cancer
    • Tumor biomarkers
    • Other

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody [C9] (ab239496)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody [C9] (ab239496)

    Paraffin-embedded human liver tissue stained for Hsp70 using ab239496 in immunohistochemical analysis.

  • Western blot - Anti-Hsp70 antibody [C9] (ab239496)
    Western blot - Anti-Hsp70 antibody [C9] (ab239496)
    All lanes : Anti-Hsp70 antibody [C9] (ab239496) at 3 µg/ml

    Lane 1 : Pig liver lysate
    Lane 2 : Pig brain lysate
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate

    Secondary
    All lanes : HRP-Linked Rabbit Anti-Mouse IgG at 1/5000 dilution

    Predicted band size: 70 kDa

  • Western blot - Anti-Hsp70 antibody [C9] (ab239496)
    Western blot - Anti-Hsp70 antibody [C9] (ab239496)
    Anti-Hsp70 antibody [C9] (ab239496) at 3 µg/ml + Recombinant human Hsp70 protein

    Secondary
    HRP-Linked Rabbit Anti-Mouse IgG at 1/5000 dilution

    Predicted band size: 70 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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