Anti-Hsp70 antibody [C9] (ab239496)
Key features and details
- Mouse monoclonal [C9] to Hsp70
- Suitable for: WB, IHC-P
- Reacts with: Human, Pig
- Isotype: IgG2b
Overview
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Product name
Anti-Hsp70 antibody [C9]
See all Hsp70 primary antibodies -
Description
Mouse monoclonal [C9] to Hsp70 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB HumanPig -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Human Hsp70 aa 250 to the C-terminus. Two N-terminal tags, His-tag and T7-tag (Expressed in E.coli).
Database link: P0DMV9 -
Positive control
- IHC-P: Human liver cancer tissue. WB: Pig liver and brain lysates; HeLa and Jurkat cell lysates; Recombinant human Hsp70 protein.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
C9 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Images
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Paraffin-embedded human liver tissue stained for Hsp70 using ab239496 in immunohistochemical analysis.
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All lanes : Anti-Hsp70 antibody [C9] (ab239496) at 3 µg/ml
Lane 1 : Pig liver lysate
Lane 2 : Pig brain lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Secondary
All lanes : HRP-Linked Rabbit Anti-Mouse IgG at 1/5000 dilution
Predicted band size: 70 kDa
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Anti-Hsp70 antibody [C9] (ab239496) at 3 µg/ml + Recombinant human Hsp70 protein
Secondary
HRP-Linked Rabbit Anti-Mouse IgG at 1/5000 dilution
Predicted band size: 70 kDa