Anti-Hsp40 antibody [3B9.E6] (ab223607)
![Anti-Hsp40 antibody [3B9.E6] (ab223607)](https://www.abcam.com/ps/products/223/ab223607/Images/ab223607-410473-anti-hsp40-antibody-3b9e6-western-blot-human-knockout-lysate.jpg "Anti-Hsp40 antibody [3B9.E6] (ab223607)")
Key features and details
- Mouse monoclonal [3B9.E6] to Hsp40
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-Hsp40 antibody [3B9.E6]
See all Hsp40 primary antibodies -
Description
Mouse monoclonal [3B9.E6] to Hsp40 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Recombinant fragment corresponding to Human Hsp40.
Database link: P25685 -
Positive control
- ICC/IF: HaCaT cells. WB: A431, A549, Daudi, HCT116, HeLa, HEK293, HepG2, HL-60, HUVEC, Jurkat, MCF7, PC-3 and T98G cell lysate; rat brain lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
3B9.E6 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-Hsp40 antibody [3B9.E6] (ab223607)All lanes : Anti-Hsp40 antibody [3B9.E6] (ab223607) at 1/500 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : DNAJB1 knockout HEK293T cell lysate
Lane 3 : A549 cell lysate
Lane 4 : Daudi cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) at 1/10000 dilution
Predicted band size: 38 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab223607 observed at 39 kDa. Red - loading control ab52901 observed at kDa.
ab223607 Anti-Hsp40 antibody [3B9.E6] was shown to specifically react with Hsp40 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266344 (knockout cell lysate ab258400) was used. Wild-type and Hsp40 knockout samples were subjected to SDS-PAGE. ab223607 and Anti-beta Tubulin [EP1331Y] - Microtubule Marker (ab52901) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunocytochemistry/ Immunofluorescence - Anti-Hsp40 antibody [3B9.E6] (ab223607)](https://www.abcam.com/ps/products/223/ab223607/Images/ab223607-283349-anti-hsp40-antibody-3b9e6-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Hsp40 antibody [3B9.E6] (ab223607)100% methanol fixed HaCat (human keratinocyte cell line) cells stained for Hsp40 (green) using ab223607 (1/100) in ICC/IF. Secondary Antibody: FITC Goat Anti-Mouse 1/50.
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![Western blot - Anti-Hsp40 antibody [3B9.E6] (ab223607)](https://www.abcam.com/ps/products/223/ab223607/Images/ab223607-283350-anti-hsp40-antibody-3b9e6-western-blot.jpg)
Western blot - Anti-Hsp40 antibody [3B9.E6] (ab223607)All lanes : Anti-Hsp40 antibody [3B9.E6] (ab223607) at 1/1000 dilution
Lane 1 : A431 cell lysate
Lane 2 : A549 cell lysate
Lane 3 : HCT116 cell lysate
Lane 4 : HeLa cell lysate
Lane 5 : HEK293 cell lysate
Lane 6 : HepG2 cell lysate
Lane 7 : HL-60 cell lysate
Lane 8 : HUVEC cell lysate
Lane 9 : Jurkat cell lysate
Lane 10 : MCF7 cell lysate
Lane 11 : PC-3 cell lysate
Lane 12 : T98G cell lysate
Lane 13 : Rat brain lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Sheep Anti-Mouse IgG HRP
Predicted band size: 38 kDa
