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Anti-FKBP52 antibody [EPR6618] - BSA and Azide free (ab240535)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6618] to FKBP52 - BSA and Azide free
  • Suitable for: ICC, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-FKBP52 antibody [EPR6618] - BSA and Azide free
    See all FKBP52 primary antibodies

  • Description

    Rabbit monoclonal [EPR6618] to FKBP52 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, WB, IHC-Pmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, Jurkat, K562, and MCF7 cell lysates; Human colon and kidney tissues.

  • General notes

    Ab240535 is the carrier-free version of ab129097. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab240535 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Western blot - Anti-FKBP52 antibody [EPR6618] - BSA and Azide free (ab240535)
    Western blot - Anti-FKBP52 antibody [EPR6618] - BSA and Azide free (ab240535)
    All lanes : Anti-FKBP52 antibody [EPR6618] (ab129097) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : K562 cell lysate
    Lane 4 : MCF7 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 52 kDa



    This data was developed using ab129097, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-FKBP52 antibody [EPR6618] - BSA and Azide free (ab240535)
    Immunocytochemistry - Anti-FKBP52 antibody [EPR6618] - BSA and Azide free (ab240535)
    This data was developed using ab129097, the same antibody clone in a different buffer formulation.ab129097 staining FKBP52 in MCF-7 (human breast carcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain and the negative control was PBS only.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP52 antibody [EPR6618] - BSA and Azide free (ab240535)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP52 antibody [EPR6618] - BSA and Azide free (ab240535)
    This data was developed using ab129097, the same antibody clone in a different buffer formulation.ab129097, at a 1/250 dilution, staining FKBP52 in paraffin embedded Human colon tissue by Immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP52 antibody [EPR6618] - BSA and Azide free (ab240535)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP52 antibody [EPR6618] - BSA and Azide free (ab240535)
    This data was developed using ab129097, the same antibody clone in a different buffer formulation.ab129097, at a 1/250 dilution, staining FKBP52 in paraffin embedded Human kidney tissue by Immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • OI-RD Scanning - Anti-FKBP52 antibody [EPR6618] - BSA and Azide free (ab240535)
    OI-RD Scanning - Anti-FKBP52 antibody [EPR6618] - BSA and Azide free (ab240535)
    This data was developed using ab129097, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-FKBP52 antibody [EPR6618] - BSA and Azide free (ab240535)
    Anti-FKBP52 antibody [EPR6618] - BSA and Azide free (ab240535)

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