Anti-Hsp27 antibody (ab223566)
Key features and details
- Rabbit polyclonal to Hsp27
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Hsp27 antibody
See all Hsp27 primary antibodies -
Description
Rabbit polyclonal to Hsp27 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Recombinant fragment (His-tag) corresponding to Human Hsp27.
Database link: P04792 -
Positive control
- WB: HeLa whole cell lysate. ICC/IF: Heat shocked HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 antibody (ab223566)
2% formaldehyde-fixed heat shocked HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Hsp27 (green) using ab223566 at 1/250 dilution in ICC/IF. Secondary: Goat Anti-Rabbit FITC at 1/200 dilution for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1/40000 for 2 hours RT. Heat shocked at 42°C for 1h.
(A) DAPI nuclear stain (B) ab223566 (C) Merge.
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Western blot - Anti-Hsp27 antibody (ab223566)Anti-Hsp27 antibody (ab223566) at 1/2000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 15 µg
Secondary
Donkey Anti-Rabbit IgG HRP
Predicted band size: 23 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?Blocking buffer: 1.5% BSA.
Primary antibody was incubated for 2 hours, the secondary for 1 hour at RT.
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Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 antibody (ab223566)2% formaldehyde-fixed heat shocked HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Hsp27 (red) using ab223566 at 1/250 dilution in ICC/IF. Secondary: Goat Anti-Rabbit APC at 1/200 dilution for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1/40000 for 2 hours RT. Heat shocked at 42°C for 1h.
(A) DAPI nuclear stain (B) ab223566 (C) Merge.
