Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR7278] to Hsp27 (phospho S82)
Suitable for: WB, IP, Dot blot
Reacts with: Mouse, Rat, Human

Product name

Anti-Hsp27 (phospho S82) antibody [EPR7278]
See all Hsp27 primary antibodies
Description

Rabbit monoclonal [EPR7278] to Hsp27 (phospho S82)
Host species

Rabbit
Specificity

ab155987 only detects Hsp27 phosphorylated at Serine 82.
Tested Applications & Species

Application Species
IP
Human

WB
Mouse

Human

See all applications and species data
Immunogen

Synthetic peptide within Human Hsp27. The exact sequence is proprietary.
Positive control
- HeLa cell lysate treated with heat shock (44°C); Human skeletal muscle and urinary bladder transitional carcinoma tissues.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
IP	Human
WB	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20ºC.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR7278
Isotype

IgG
Research areas

Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987)

All lanes : Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987) at 1/1000 dilution

Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) with 44°C heat shock whole cell lysates
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution

Predicted band size: 23 kDa
Observed band size: 27 kDa
why is the actual band size different from the predicted?

Exposure time: 1 minute

Blocking and diluting buffer: 5% NFDM/TBST.
Dot Blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987)

Dot blot analysis of Lane 1: Hsp27 (pS82) phospho peptide and Lane 2: Hsp27 non-phospho peptide, labeling Hsp27 (phospho S82) with ab155987 at 1/1000 dilution. 5% NFDM/TBST was used as the blocking and diluting buffer. ab97051, a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody was used at 1/100000 dilution. Exposure time: 10 seconds.
Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987)

All lanes : Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987) at 1/1500 dilution (purified)

Lane 1 : Mouse heart
Lane 2 : Rat heart

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 23 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987)

Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987) at 1/1500 dilution (purified) + HeLa cell lysate treated with heat shock (44°C) at 10 µg

Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 23 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunoprecipitation - Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987)

ab155987 (purified) at 1/75 immunoprecipitating Hsp27 (phospho S82) in HeLa cell lysate treated with heat shock (44°C) (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987)

All lanes : Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987) at 1/200 dilution (unpurified)

Lane 1 : Mouse heart
Lane 2 : Rat heart

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 23 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987)

Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987) at 1/200 dilution (unpurified) + HeLa cells treated with heat shock (44°C) at 10 µg

Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 23 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Western blot - Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987)

All lanes : Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987) at 1/1000 dilution (unpurified)

Lane 1 : Untreated HeLa cell lysate
Lane 2 : HeLa cell lysate treated with heat shock (44°C).

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 23 kDa
Immunoprecipitation - Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987)

ab155987 (unpurified) at 1/75 immunoprecipitating Hsp27 (phospho S82) in HeLa cell lysate treated with heat shock (44°C) (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987)

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