Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸ 10 µl 120 614 ₸

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Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP1727Y] to Superoxide Dismutase 1
Suitable for: WB, Flow Cyt, IHC-P, ICC/IF
Reacts with: Mouse, Rat, Human

Product name

Anti-Superoxide Dismutase 1 antibody [EP1727Y]
See all Superoxide Dismutase 1 primary antibodies
Description

Rabbit monoclonal [EP1727Y] to Superoxide Dismutase 1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC	Mouse Rat
ICC/IF	Human
IHC-P	Mouse Rat Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide within Human Superoxide Dismutase 1 aa 100-200. The exact sequence is proprietary.
Positive control
- IHC-P: Mouse cerebrum, rat liver and human breast carcinoma tissue WB: Jurkat cell lysate; Mouse and rat brain tissue lysates. ICC/IF: HeLa, PC-12 and NIH/3T3 cells. Flow Cyt: HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EP1727Y
Isotype

IgG
Research areas

Immunocytochemistry - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 cells labelling Superoxide Dismutase 1 with ab51254 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic and weak nuclear staining in NIH/3T3 cell line ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 2.5 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1000 2 ug/ml dilution.
Immunocytochemistry - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized PC-12 cells labelling Superoxide Dismutase 1 with ab51254 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in PC-12 cell line ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 2.5 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1000 2 ug/ml dilution.
Western blot - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)

All lanes : Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254) at 0.015 µg/ml

Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml

Predicted band size: 16 kDa
Observed band size: 18 kDa
why is the actual band size different from the predicted?

Blocking/diluting Buffer and concentration: 5% NFDM /TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebrum tissue sections labeling Superoxide Dismutase 1 with purified ab51254 at 1/1000 dilution (0.15 µg/ml).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: secondary antibody only control. Hematoxylin was used as a counterstain.
Flow Cytometry - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)

Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Superoxide Dismutase 1 with ab51254 at 1/20 dilution (1 µg) (Red). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were fixed with 4% paraformaldehyde. Rabbit monoclonal IgG (ab172730) was used as isotype control (Black). Unlabelled control: Cells without incubation with primary antibody and secondary antibody (Blue).
Western blot - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)

Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254) at 1/50000 dilution + Jurkat cell lysate at 10 µg

Secondary
Goat anti-rabbit HRP labeled at 1/2000 dilution

Predicted band size: 16 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue sections labeling Superoxide Dismutase 1 with purified ab51254 at 1/1000 dilution (0.15 µg/ml).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: secondary antibody only control. Hematoxylin was used as a counterstain.
Immunocytochemistry/ Immunofluorescence - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)

Immunofluorescent staining of HeLa cells using ab51254 (1:100).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)

Ab51254 (1:100) staining human Superoxide Dismutase 1 in human breast carcinoma tissue by immunohistochemistry using paraffin embedded tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab51254)

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