Anti-PI 3 Kinase p85 beta antibody [T15] (ab28356)
![Anti-PI 3 Kinase p85 beta antibody [T15] (ab28356)](https://www.abcam.com/ps/products/28/ab28356/Images/ab28356-409564-anti-pi-3-kinase-p85-beta-antibody-t15-western-blot-hek-293t-lysates.jpg "Anti-PI 3 Kinase p85 beta antibody [T15] (ab28356)")
Key features and details
- Mouse monoclonal [T15] to PI 3 Kinase p85 beta
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-PI 3 Kinase p85 beta antibody [T15]
See all PI 3 Kinase p85 beta primary antibodies -
Description
Mouse monoclonal [T15] to PI 3 Kinase p85 beta -
Host species
Mouse -
Specificity
T15 recognises the p85 beta subunit of bovine PI 3 kinase. It does not cross react with the p85 alpha subunit. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Balculovirus expressed (Recombinant) bovine P85 beta subunit of PI 3 kinase.
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Positive control
- WB: HEK293T cell lysate; Lysates of SF9 cells infected with baculovirus expressing p85 beta.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.09% Sodium azide -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
This antibody is purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
T15 -
Myeloma
NS1 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-PI 3 Kinase p85 beta antibody [T15] (ab28356)All lanes : Anti-PI 3 Kinase p85 beta antibody [T15] (ab28356) at 1/1000 dilution
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : PIK3R2 knockout HEK-293T cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 82 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?Lanes 1- 2: Merged signal (red and green). Green - ab28356 observed at 85 kDa. Red - Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) observed at 37 kDa.
ab28356 was shown to react with PI 3 Kinase p85 beta in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266799 (knockout cell lysate ab257586) was used. Wild-type HEK-293T and PIK3R2 knockout HEK-293T cell lysates were subjected to SDS-PAGE. ab28356 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye®800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye®680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-PI 3 Kinase p85 beta antibody [T15] (ab28356)](https://www.abcam.com/ps/products/28/ab28356/Images/ab28356-39274-anti-pi-3-kinase-p85-beta-antibody-t15-western-blot.jpg)
Western blot - Anti-PI 3 Kinase p85 beta antibody [T15] (ab28356)Western Blotting of HEK293 and HepG2 cell lysates with Mouse anti Bovine PI-3 kinase p85 subunit beta (ab28356), recombinant p85ß is included as a positive control.
