Human HSP60 ELISA Kit (ab124534)
Key features and details
- Sample type: Cell Lysate, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human HSP60 ELISA Kit
See all HSP60 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 4 2.1% Inter-assay Sample n Mean SD CV% Overall 3 6.7% -
Sample type
Tissue Extracts, Cell Lysate -
Assay type
Sandwich (quantitative) -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Product overview
ab124534 HSP60 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of HSP60 protein a 60 kDa protein encoded by the Human HSPD1 gene, in cell and tissue lysates. The assay employs an HSP60 specific antibody coated onto well plate strips.
Standards and samples are pipetted into the wells and analyte present in the sample is bound to the wells by the immobilized antibody. The wells are washed and an anti-HSP60 primary detector antibody is added. After washing away unbound primary detector antibody, HRP-conjugated secondary detector antibody specific for the primary detector antibody is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of analyte bound. The developing blue color is measured at 600 nm.
Optionally the reaction can be stopped by adding hydrochloric acid which changes the color from blue to yellow and the intensity can be measured at 450 nm.
Species– Human reactive. Non-reactive with mouse and rat. Others untested.
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Notes
Typical working ranges:
HepG2 cell extract: 0.39 - 200 µg/mL
Hela cell extract: 3.125 - 200 µg/mL
Store all components at 4°C. This kit is stable for at least 6 months from receipt. Unused microplate strips should be returned to the pouch containing the desiccant and resealed.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Blocking Buffer 1 x 6ml 10X HRP Label 1 x 1ml 10X HSP60 Detector Antibody 1 x 1ml 20X Buffer 1 x 20ml Extraction Buffer (ab260490) 1 x 15ml HSP60 Microplate 1 unit HRP Development Solution 1 x 12ml -
Research areas
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Alternative names
- Chaperonin
- Heat shock 60kDa protein 1
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Database links
- Entrez Gene: 3329 Human
- SwissProt: P10809 Human
Images
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Flow Cytometry - HSP60 Human ELISA Kit (ab124534)Analysis of HSP60 staining in Hela cells by Flow: blue: 1µg/mL HSP60 antibody; red: an equal amount of an isotype control antibody.
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Immunocytochemistry/ Immunofluorescence - HSP60 Human ELISA Kit (ab124534)Analysis of HSP60 staining in HDFn cells by ICC: HSP60 primary antibody at 1 µg/mL (green). -
Western blot - HSP60 Human ELISA Kit (ab124534)Western blot using the HSP60 detector antibody at 1µg/mL demonstrates assay specificity. HepG2 cell lysate loaded at 15µg/lane. -
Sandwich ELISA - HSP60 Human ELISA Kit (ab124534)Sensitivity of HSP60 ELISA kit is used to demonstrate the quantitative differentiation among different human cell lines. -
Sandwich ELISA - HSP60 Human ELISA Kit (ab124534)Detection of endogenous HSP60 levels in different cell lysates using HSP60 ELISA kit. -
Western blot - HSP60 Human ELISA Kit (ab124534)Detection of endogenous HSP60 levels in different cell lysates using Western blot. HSP60 antibody at 1µg/mL: HepG2 cell lysate (lane 2), HeLa cell lysate (lane 3), and HDFn lysate (lane 4), each were loaded at 15µg/lane. -
Sandwich ELISA - HSP60 Human ELISA Kit (ab124534)Example standard curve of serially titrated Hela cell lysate in the working range of the assay. -
Sandwich ELISA - HSP60 Human ELISA Kit (ab124534)Example standard curve of serially titrated HepG2 cell lysate in the working range of the assay.