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Signal Transduction Protein Trafficking Chaperones Heat Shock Proteins

Anti-Hsp27 (phospho S15) antibody (ab131340)

Anti-Hsp27 (phospho S15) antibody (ab131340)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Hsp27 (phospho S15)
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Hsp27 (phospho S15) antibody
    See all Hsp27 primary antibodies
  • Description

    Rabbit polyclonal to Hsp27 (phospho S15)
  • Host species

    Rabbit
  • Specificity

    ab131340 detects Hsp27 only when phosphorylated at serine 15
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a sequence surrounding phosphorylation site of serine 15 (

    G-P-S(p)-W-D

    ) derived from Human Hsp27 (NP_001531.1).
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • HeLa cells, HeLa cell extracts, Human breast carcinoma tissue
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 49% PBS

    PBS is without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab131340 is purified by affinity-chromatography using an epitode-specific phosphopetide. Non-phoso specific antibodies are removed by chromatography using a non-phosphopeptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Chaperones
    • Heat Shock Proteins
    • Cancer
    • Tumor biomarkers
    • Other
    • Cardiovascular
    • Atherosclerosis
    • Ischemia / Reperfusion
    • Cardiovascular
    • Heart
    • Contractility
    • Contractile Proteins
    • Actins

Images

  • Western blot - Anti-Hsp27 (phospho S15) antibody (ab131340)
    Western blot - Anti-Hsp27 (phospho S15) antibody (ab131340)
    All lanes : Anti-Hsp27 (phospho S15) antibody (ab131340) at 1/500 dilution

    Lane 1 : HeLa cell extracts
    Lane 2 : HeLa cell extracts treated with UV

    Predicted band size: 23 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 (phospho S15) antibody (ab131340)
    Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 (phospho S15) antibody (ab131340)
    Immunofluorescence analysis of methanol-fixed HeLa cells using ab131340 at a 1/100 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 (phospho S15) antibody (ab131340)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 (phospho S15) antibody (ab131340)
    Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue using ab131340 at a 1/50 dilution, in the absence (left) and presence (right) of the blocking peptide.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Hsp27 (phospho S15) antibody (ab131340)

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    Alexa Fluor® 594 Anti-Hsp27 antibody [EPR5477] (ab215328)

    Applications: Flow Cyt, ICC/IF

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    Anti-Hsp27 antibody [EPR5477] (ab109376)

    Applications: Flow Cyt, ICC/IF, WB

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    Anti-Hsp27 (phospho S78) antibody [Y175] (ab32501)

    Applications: Dot, ICC/IF, IHC-P, WB

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    Anti-Hsp27 (phospho S15) antibody [EP2293Y] (ab76313)

    Applications: ICC/IF, WB

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    Anti-Hsp27 (phospho S82) antibody [EPR7278] (ab155987)

    Applications: Dot, IP, WB

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    Alexa Fluor® 647 Anti-Hsp27 antibody [EPR5477] (ab194078)

    Applications: Flow Cyt, ICC/IF

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    Anti-Hsp27 antibody [EPR5477] - BSA and Azide free (ab229442)

    Applications: Flow Cyt, ICC/IF, WB

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