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Signal Transduction Protein Trafficking Chaperones Heat Shock Proteins

Anti-Hsp27 antibody (ab12351)

Price and availability

301 536 ₸

Availability

Order now and get it on Thursday February 25, 2021

Anti-Hsp27 antibody (ab12351)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Hsp27
  • Suitable for: IHC, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Hsp27 antibody
    See all Hsp27 primary antibodies
  • Description

    Rabbit polyclonal to Hsp27
  • Host species

    Rabbit
  • Specificity

    This antibody detects an 25 kDa protein, corresponding to the apparent molecular mass of Heat Shock Protein 27 (Hsp27) on SDS-PAGE immunoblots. This antibody has been shown to react with both the phosphorylated and the non-phosphorylated forms of Hsp27. Note: It has been reported that certain murine cell lines do not express Hsp27 under certain conditions. This antibody also recognizes a mitochondrial small Hsp (35 kDa) in rat PC12 cells. This mitochondrial small Hsp is proposed to protect mitochondrial complex I from oxidative stress.
  • Tested applications

    Suitable for: IHC, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Pig, Monkey
  • Immunogen

    Recombinant full length protein corresponding to Human Hsp27.
    Database link: P04792

  • General notes

    For maximum product recovery, after thawing, centrifuge the product vial before removing cap.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Chaperones
    • Heat Shock Proteins
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Assembly
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microtubules
    • Tubulin
    • Cancer
    • Tumor biomarkers
    • Other
    • Cardiovascular
    • Atherosclerosis
    • Ischemia / Reperfusion
    • Cardiovascular
    • Heart
    • Contractility
    • Contractile Proteins
    • Actins

Images

  • Western blot - Anti-Hsp27 antibody (ab12351)
    Western blot - Anti-Hsp27 antibody (ab12351)
    All lanes : Anti-Hsp27 antibody (ab12351) at 1 µg/ml

    Lane 1 : MW Marker
    Lane 2 : HSP27 Recombinant Human Protein
    Lane 3 : HSP25 Recombinant Murine Protein (Negative Control)
    Lane 4 : HeLa cell lysate
    Lane 5 : HeLa cell lysate, Heat Shocked
    Lane 6 : Vero, Heat Shocked
    Lane 7 : 3T3 cell lysate, heat shocked
    Lane 8 : PC-12 cell lysate, Heat Shocked

    Predicted band size: 25 kDa

  • Immunohistochemistry - Anti-Hsp27 antibody (ab12351)
    Immunohistochemistry - Anti-Hsp27 antibody (ab12351)

    Immunohistochemical analysis of human heart tissue sections with ab12351 at 10 µg/mL.

  • Western blot - Anti-Hsp27 antibody (ab12351)
    Western blot - Anti-Hsp27 antibody (ab12351) This image is courtesy of an anonymous Abreview
    Anti-Hsp27 antibody (ab12351) at 1/500 dilution + Rat keratinocytes, whole cell lysate at 10 µg

    Secondary
    HRP conjugated goat anti-rabbit antibody

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 25 kDa
    Observed band size: 25 kDa
    Additional bands at: 120 kDa (possible non-specific binding), 215 kDa (possible non-specific binding)


    Exposure time: 5 minutes

    See Abreview

  • Western blot - Anti-Hsp27 antibody (ab12351)
    Western blot - Anti-Hsp27 antibody (ab12351)
    All lanes : Anti-Hsp27 antibody (ab12351) at 1/1000 dilution

    Lane 1 : Molecular weight marker
    Lane 2 : Cell lysates prepared from untreated PC-12 cells
    Lane 3 : Cell lysates prepared from heat shock treated PC-12 cells

    Predicted band size: 25 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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