Anti-Hsp27 antibody (ab12351)
Key features and details
- Rabbit polyclonal to Hsp27
- Suitable for: IHC, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Hsp27 antibody
See all Hsp27 primary antibodies -
Description
Rabbit polyclonal to Hsp27 -
Host species
Rabbit -
Specificity
This antibody detects an 25 kDa protein, corresponding to the apparent molecular mass of Heat Shock Protein 27 (Hsp27) on SDS-PAGE immunoblots. This antibody has been shown to react with both the phosphorylated and the non-phosphorylated forms of Hsp27. Note: It has been reported that certain murine cell lines do not express Hsp27 under certain conditions. This antibody also recognizes a mitochondrial small Hsp (35 kDa) in rat PC12 cells. This mitochondrial small Hsp is proposed to protect mitochondrial complex I from oxidative stress. -
Tested applications
Suitable for: IHC, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Pig, Monkey -
Immunogen
Recombinant full length protein corresponding to Human Hsp27.
Database link: P04792 -
General notes
For maximum product recovery, after thawing, centrifuge the product vial before removing cap.Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Hsp27 antibody (ab12351) at 1 µg/ml
Lane 1 : MW Marker
Lane 2 : HSP27 Recombinant Human Protein
Lane 3 : HSP25 Recombinant Murine Protein (Negative Control)
Lane 4 : HeLa cell lysate
Lane 5 : HeLa cell lysate, Heat Shocked
Lane 6 : Vero, Heat Shocked
Lane 7 : 3T3 cell lysate, heat shocked
Lane 8 : PC-12 cell lysate, Heat Shocked
Predicted band size: 25 kDa
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Immunohistochemical analysis of human heart tissue sections with ab12351 at 10 µg/mL.
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Anti-Hsp27 antibody (ab12351) at 1/500 dilution + Rat keratinocytes, whole cell lysate at 10 µg
Secondary
HRP conjugated goat anti-rabbit antibody
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 25 kDa
Observed band size: 25 kDa
Additional bands at: 120 kDa (possible non-specific binding), 215 kDa (possible non-specific binding)
Exposure time: 5 minutes
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All lanes : Anti-Hsp27 antibody (ab12351) at 1/1000 dilution
Lane 1 : Molecular weight marker
Lane 2 : Cell lysates prepared from untreated PC-12 cells
Lane 3 : Cell lysates prepared from heat shock treated PC-12 cells
Predicted band size: 25 kDa