Recombinant mouse Alanine Transaminase protein (Active) (ab222953)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: MS, Functional Studies, SDS-PAGE
-
Product name
Recombinant mouse Alanine Transaminase protein (Active)
See all Alanine Transaminase proteins and peptides -
Biological activity
Specific activity is > 40 units/mg, and is defined as the amount of enzyme that converts 1 µmole of L-Alanine to L-Glutamate per minute at pH 7.5 at 37°C.
-
Purity
> 90 % SDS-PAGE.
ab222953 was purified using conventional chromatography techniques. -
Expression system
Escherichia coli -
Accession
-
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
-
Species
Mouse -
Sequence
MGSSHHHHHHSSGLVPRGSHMGSMASQRNDRIQASRNGLKGKVLTLDTMN PCVRRVEYAVRGPIVQRALELEQELRQGVKKPFTEVIRANIGDAQAMGQR PITFFRQVLALCVYPNLLSSPDFPEDAKRRAERILQACGGHSLGAYSISS GIQPIREDVAQYIERRDGGIPADPNNIFLSTGASDAIVTMLKLLVAGEGR ARTGVLIPIPQYPLYSAALAELDAVQVDYYLDEERAWALDIAELRRALCQ ARDRCCPRVLCVINPGNPTGQVQTRECIEAVIRFAFEEGLFLMADEVYQD NVYAEGSQFHSFKKVLTEMGPPYATQQELASFHSVSKGYMGECGFRGGYV EVVNMDAEVQKQMAKLMSVRLCPPVPGQALMGMVVSPPTPSEPSFKQFQA ERQEVLAELAAKAKLTEQVFNEAPGIRCNPVQGAMYSFPQIQLPLKAVQR AQDLGLAPDMFFCLCLLEETGICVVPGSGFGQQEGTYHFRMTILPPMEKL RVLLEKLRHFHAKFTHEYS -
Predicted molecular weight
58 kDa including tags -
Amino acids
1 to 496 -
Tags
His tag N-Terminus -
Additional sequence information
NP_877957.
-
Preparation and Storage
-
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 0.02% DTT, 10% Glycerol (glycerin, glycerine), PBSThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
-
SDS-PAGE - Recombinant mouse Alanine Transaminase protein (Active) (ab222953)
15% SDS-PAGE analysis of 3 µg ab222953.
