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Neuroscience Neurology process Growth and Development Axonal Guidance Proteins

Anti-p92 antibody (ab72210)

Anti-p92 antibody (ab72210)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to p92
  • Suitable for: WB
  • Reacts with: Mouse
  • Isotype: IgG

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Overview

  • Product name

    Anti-p92 antibody
  • Description

    Rabbit polyclonal to p92
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Mouse
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Mouse p92 aa 750 to the C-terminus conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab87446)

  • General notes

    This product may be covered by one or more of the following U.S. Patents.

    6,166,192
    6,426,411
    6,525,178
    6,872,391
    6,908,987
    7,250,283
    7,091,006
    7,354,738
    8,105,774
    8,445,233

     

    Previously labelled as Advillin. 

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Growth and Development
    • Axonal Guidance Proteins
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Developmental Biology
    • Organogenesis
    • Nervous system development

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab72210 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Mouse
All applications
Rat
Human
Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 92 kDa (predicted molecular weight: 92 kDa).
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 92 kDa (predicted molecular weight: 92 kDa).

Target

  • Function

    Ca(2+)-regulated actin-binding protein. May have a unique function in the morphogenesis of neuronal cells which form ganglia. Required for SREC1-mediated regulation of neurite-like outgrowth. Plays a role in regenerative sensory axon outgrowth and remodeling processes after peripheral injury in neonates. Involved in the formation of long fine actin-containing filopodia-like structures in fibroblast. Plays a role in ciliogenesis.
  • Tissue specificity

    Most highly expressed in the small intestine and colonic lining. Weaker expression also detected in the thymus, prostate, testes and uterus.
  • Sequence similarities

    Belongs to the villin/gelsolin family.
    Contains 6 gelsolin-like repeats.
    Contains 1 HP (headpiece) domain.
  • Cellular localization

    Cytoplasm > cytoskeleton. Cell projection. Cell projection > axon.
  • Target information above from: UniProt accession O75366 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 10677 Human
    • Entrez Gene: 11567 Mouse
    • Entrez Gene: 79253 Rat
    • Omim: 613397 Human
    • SwissProt: O75366 Human
    • SwissProt: O88398 Mouse
    • SwissProt: Q9WU06 Rat
    • Unigene: 584854 Human
    • Unigene: 10739 Mouse
    • Unigene: 20161 Rat
    see all
  • Alternative names

    • Actin binding protein DOC 6 antibody
    • Actin binding protein DOC6 antibody
    • Advil antibody
    • Advillin antibody
    • AVIL antibody
    • AVIL_HUMAN antibody
    • DOC 6 antibody
    • DOC6 antibody
    • p92 antibody
    see all

Images

  • Western blot - Anti-p92 antibody (ab72210)
    Western blot - Anti-p92 antibody (ab72210)
    All lanes : Anti-p92 antibody (ab72210) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Mouse skin tissue lysate - total protein (ab4025)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 92 kDa
    Observed band size: 92 kDa
    Additional bands at: 105 kDa, 52 kDa. We are unsure as to the identity of these extra bands.

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (3)

    Publishing research using ab72210? Please let us know so that we can cite the reference in this datasheet.

    ab72210 has been referenced in 3 publications.

    • Esmaeilniakooshkghazi A  et al. Mouse intestinal tuft cells express advillin but not villin. Sci Rep 10:8877 (2020). PubMed: 32483224
    • Xie Z  et al. A cytoskeleton regulator AVIL drives tumorigenesis in glioblastoma. Nat Commun 11:3457 (2020). PubMed: 32651364
    • Nonomura K  et al. Piezo2 senses airway stretch and mediates lung inflation-induced apnoea. Nature 541:176-181 (2017). PubMed: 28002412

    Images

    • Western blot - Anti-p92 antibody (ab72210)
      Western blot - Anti-p92 antibody (ab72210)
      All lanes : Anti-p92 antibody (ab72210) at 1 µg/ml

      Lane 1 : Brain (Mouse) Tissue Lysate
      Lane 2 : Mouse skin tissue lysate - total protein (ab4025)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 92 kDa
      Observed band size: 92 kDa
      Additional bands at: 105 kDa, 52 kDa. We are unsure as to the identity of these extra bands.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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