Anti-Hsp27 (phospho S78) antibody [Y175] (ab32501)
![Anti-Hsp27 (phospho S78) antibody [Y175] (ab32501)](https://www.abcam.com/ps/products/32/ab32501/Images/ab32501-278134-anti-hsp27-phospho-s78-antibody-y175-western-blot.jpg "Anti-Hsp27 (phospho S78) antibody [Y175] (ab32501)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y175] to Hsp27 (phospho S78)
- Suitable for: WB, IHC-P, ICC/IF, Dot blot
- Reacts with: Human
Overview
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Product name
Anti-Hsp27 (phospho S78) antibody [Y175]
See all Hsp27 primary antibodies -
Description
Rabbit monoclonal [Y175] to Hsp27 (phospho S78) -
Host species
Rabbit -
Specificity
ab32501 recognises Hsp27 (phospho S78). The antibody will detect Src phosphorylation on Serine 78. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
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Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Serine 78 of human HSP27
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Positive control
- HeLa cells, human breast carcinoma
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
Y175 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Hsp27 (phospho S78) antibody [Y175] (ab32501)All lanes : Anti-Hsp27 (phospho S78) antibody [Y175] (ab32501) at 1/5000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysates
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysates, treated with anisomycin.
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysates, treated with anisomycin. Then the membrane was incubated with phosphatase.
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Predicted band size: 23 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?Blocking buffer 5% NFDM/TBST
Diluting buffer 5% NFDM/TBST
Secondary Ab at 1/20,000 dilution
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![Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 (phospho S78) antibody [Y175] (ab32501)](https://www.abcam.com/ps/products/32/ab32501/Images/ab32501-278137-anti-hsp27-phospho-s78-antibody-y175-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 (phospho S78) antibody [Y175] (ab32501)ab32501 staining Hsp27 in HeLa cells by Immunocytochemistry. Tissue was fixed with 4% paraformaldehyde. Samples were incubated with primary antibody (4.5 μg/ml). ab150077 AlexaFluor®488 Goat anti-Rabbit (1/1000) was used as the secondary antibody. Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 2.5 μg/ml and DAPI were used as counter stains.
Confocal image showing the expression was increased after treatment with anisomycin (25ug/ml for 30min) and then decreased after treatment with the Lambda Protein Phosphatase 31℃ for 2h
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![Dot Blot - Anti-Hsp27 (phospho S78) antibody [Y175] (ab32501)](https://www.abcam.com/ps/products/32/ab32501/Images/ab32501-45044-anti-hsp27-phospho-s78-antibody-y175-dot-blot.jpg)
Dot Blot - Anti-Hsp27 (phospho S78) antibody [Y175] (ab32501)Dot Blot analysis on immunogen phospho-peptide (A) and non-phospho peptide (B) using ab32501 at dilution 1/2000. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 (phospho S78) antibody [Y175] (ab32501)](https://www.abcam.com/ps/products/32/ab32501/Images/ab32501-45043-anti-hsp27-phospho-s78-antibody-y175-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 (phospho S78) antibody [Y175] (ab32501)Immunohistochemical analysis of Hsp27 phospho S78 expression in paraffin embedded human breast carcinoma using 1/250 ab32501.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 (phospho S78) antibody [Y175] (ab32501)](https://www.abcam.com/ps/products/32/ab32501/Images/ab32501-45042-anti-hsp27-phospho-s78-antibody-y175-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 (phospho S78) antibody [Y175] (ab32501)Immunofluorescent analysis of Hsp27 phospho S78 expression in HeLa cells using 1/250 ab32501. -
![Anti-Hsp27 (phospho S78) antibody [Y175] (ab32501)](https://www.abcam.com/ps/products/32/ab32501/Images/ab32501-7-benefits-of-recombinant-antibodies.png)
Anti-Hsp27 (phospho S78) antibody [Y175] (ab32501)
