Anti-Hsp75 antibody [EPR5382] - BSA and Azide free (ab248218)
![Anti-Hsp75 antibody [EPR5382] - BSA and Azide free (ab248218)](https://www.abcam.com/ps/products/248/ab248218/Images/ab248218-403559-anti-hsp75-antibody-epr5382-bsa-and-azide-free-western-blot.jpg "Anti-Hsp75 antibody [EPR5382] - BSA and Azide free (ab248218)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5382] to Hsp75 - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-Hsp75 antibody [EPR5382] - BSA and Azide free
See all Hsp75 primary antibodies -
Description
Rabbit monoclonal [EPR5382] to Hsp75 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab248218 is the carrier-free version of ab128914 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab248218 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR5382 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Hsp75 antibody [EPR5382] - BSA and Azide free (ab248218)All lanes : Anti-Hsp75 antibody [EPR5382] (ab128914) at 1/1000 dilution
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate prepared using RIPA lysis method
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate prepared using 1%SDS hot lysis method
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/20000 dilution
Predicted band size: 80 kDa
Exposure time: 3 minutesThis data was developed using ab128914, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
For this product, we recommend 1% SDS hot lysis method.
> For lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy). -
![Western blot - Anti-Hsp75 antibody [EPR5382] - BSA and Azide free (ab248218)](https://www.abcam.com/ps/products/248/ab248218/Images/ab248218-399031-anti-hsp75-antibody-epr5382-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Hsp75 antibody [EPR5382] - BSA and Azide free (ab248218)All lanes : Anti-Hsp75 antibody [EPR5382] (ab128914) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Hela cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution
Predicted band size: 80 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?This data was developed using ab128914, the same antibody clone in a different buffer formulation.
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![Anti-Hsp75 antibody [EPR5382] - BSA and Azide free (ab248218)](https://www.abcam.com/ps/products/248/ab248218/Images/ab248218-3-benefits-of-recombinant-antibodies.png)
Anti-Hsp75 antibody [EPR5382] - BSA and Azide free (ab248218)