Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Peroxiredoxin 6 antibody [EPR3754] - BSA and Azide free (ab240057)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3754] to Peroxiredoxin 6 - BSA and Azide free
Suitable for: IHC-P, WB
Knockout validated
Reacts with: Mouse, Rat, Human

Product name

Anti-Peroxiredoxin 6 antibody [EPR3754] - BSA and Azide free
See all Peroxiredoxin 6 primary antibodies
Description

Rabbit monoclonal [EPR3754] to Peroxiredoxin 6 - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: IHC-P, WBmore details
Unsuitable for: IP
Species reactivity

Reacts with: Mouse, Rat, Human
Predicted to work with: Pig
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
General notes
Ab240057 is the carrier-free version of ab133348. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab240057 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR3754
Isotype

IgG
Research areas

Western blot - Anti-Peroxiredoxin 6 antibody [EPR3754] - BSA and Azide free (ab240057)

All lanes : Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348) at 1/1000 dilution

Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : PRDX6 (Peroxiredoxin 6) knockout HAP1 whole cell lysate
Lane 3 : A431 whole cell lysate
Lane 4 : PC3 whole cell lysate

Lysates/proteins at 20 µg per lane.

Predicted band size: 25 kDa

Lanes 1 - 4: Merged signal (red and green). Green - ab133348 observed at 25 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab133348 was shown to specifically react with Peroxiredoxin 6 in wild-type HAP1 cells as signal was lost in PRDX6 (Peroxiredoxin 6) knockout cells. Wild-type and PRDX6 (Peroxiredoxin 6) knockout samples were subjected to SDS-PAGE. Ab133348 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 6 antibody [EPR3754] - BSA and Azide free (ab240057)

Immunohistochemical analysis of paraffin-embedded Human brain tissue labelling Peroxiredoxin 6 with ab133348 at 1/250 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133348).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 6 antibody [EPR3754] - BSA and Azide free (ab240057)

Immunohistochemical analysis of paraffin embedded normal Human liver tissue using ab133348 showing +ve staining.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133348).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 6 antibody [EPR3754] - BSA and Azide free (ab240057)

Immunohistochemical analysis of paraffin embedded normal Human placenta tissue using ab133348 showing +ve staining.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133348).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 6 antibody [EPR3754] - BSA and Azide free (ab240057)

Immunohistochemical analysis of paraffin embedded normal Human stomach tissue using ab133348 showing +ve staining.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133348).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 6 antibody [EPR3754] - BSA and Azide free (ab240057)

Immunohistochemical analysis of paraffin embedded normal Human testis tissue using ab133348 showing +ve staining.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133348).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-Peroxiredoxin 6 antibody [EPR3754] - BSA and Azide free (ab240057)

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