Anti-Peroxiredoxin 6 antibody (ab73683)
Key features and details
- Rabbit polyclonal to Peroxiredoxin 6
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Peroxiredoxin 6 antibody
See all Peroxiredoxin 6 primary antibodies -
Description
Rabbit polyclonal to Peroxiredoxin 6 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Cow, Pig, Non human primates
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Immunogen
Synthetic peptide corresponding to Human Peroxiredoxin 6 aa 150 to the C-terminus (internal sequence) conjugated to keyhole limpet haemocyanin.
(Peptide available asab73682) -
Positive control
- This antibody gave a positive signal in the following lysates: Liver (Mouse) Tissue, Lung (Rat) Tissue, HeLa Nuclear , Hela Whole Cell - Bleomycin Treated (40U/ml), Thymus (Rat) Tissue, Kidney (Mouse) Tissue, HepG2 Whole Cell
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab73683 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes IHC-P Use a concentration of 1 µg/ml. WB Use a concentration of 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa). ICC/IF Use a concentration of 1 µg/ml. Target
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Function
Involved in redox regulation of the cell. Can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. May play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. -
Sequence similarities
Belongs to the ahpC/TSA family. Rehydrin subfamily.
Contains 1 thioredoxin domain. -
Cellular localization
Cytoplasm. Lysosome. Cytoplasmic vesicle. Also found in lung secretory organelles. - Information by UniProt
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Database links
- Entrez Gene: 9588 Human
- Entrez Gene: 11758 Mouse
- Entrez Gene: 399538 Pig
- Entrez Gene: 94167 Rat
- Omim: 602316 Human
- SwissProt: P30041 Human
- SwissProt: O08709 Mouse
- SwissProt: Q9TSX9 Pig
see all -
Alternative names
- 1 Cys antibody
- 1 Cys peroxiredoxin antibody
- 1 Cys PRX antibody
see all
Images
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Western blot - Anti-Peroxiredoxin 6 antibody (ab73683)All lanes : Anti-Peroxiredoxin 6 antibody (ab73683) at 1 µg/ml
Lane 1 : Liver (Mouse) Tissue Lysate
Lane 2 : Lung (Rat) Tissue Lysate
Lane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 4 : Hela Whole Cell Lysate - Bleomycin Treated (40U/ml)
Lane 5 : Thymus (Rat) Tissue Lysate
Lane 6 : Kidney (Mouse) Tissue Lysate
Lane 7 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 25 kDa
Observed band size: 25 kDa
Additional bands at: 30 kDa, 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute -
Immunocytochemistry/ Immunofluorescence - Anti-Peroxiredoxin 6 antibody (ab73683)ICC/IF image of ab73683 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab73683, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa, HepG2 and MCF7 cells at 1µg/ml. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 6 antibody (ab73683)IHC image of Peroxiredoxin 6 staining in Human Hippocampus FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab73683, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
Protocols
Datasheets and documents
References (0)
ab73683 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-Peroxiredoxin 6 antibody (ab73683)All lanes : Anti-Peroxiredoxin 6 antibody (ab73683) at 1 µg/ml
Lane 1 : Liver (Mouse) Tissue Lysate
Lane 2 : Lung (Rat) Tissue Lysate
Lane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 4 : Hela Whole Cell Lysate - Bleomycin Treated (40U/ml)
Lane 5 : Thymus (Rat) Tissue Lysate
Lane 6 : Kidney (Mouse) Tissue Lysate
Lane 7 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 25 kDa
Observed band size: 25 kDa
Additional bands at: 30 kDa, 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
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Immunocytochemistry/ Immunofluorescence - Anti-Peroxiredoxin 6 antibody (ab73683)ICC/IF image of ab73683 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab73683, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa, HepG2 and MCF7 cells at 1µg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 6 antibody (ab73683)IHC image of Peroxiredoxin 6 staining in Human Hippocampus FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab73683, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
