Anti-GPAT4 antibody (ab76707)
Key features and details
- Rabbit polyclonal to GPAT4
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GPAT4 antibody
See all GPAT4 primary antibodies -
Description
Rabbit polyclonal to GPAT4 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human GPAT4 aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab91636) -
Positive control
- This antibody gave a positive signal in human skeletal muscle tissue lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76707 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseRatCowPigOrangutanApplication Abreviews Notes WB Notes Application notesWB: Use at a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.Target
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Function
Esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipid biosynthesis. Active against both saturated and unsaturated long-chain fatty acyl-CoAs. -
Tissue specificity
Ubiquitous. Relatively high level of expression in skeletal muscle, heart and testis. Relatively low level of expression in lung. -
Pathway
Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3. -
Sequence similarities
Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family. -
Domain
The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 137964 Human
- Entrez Gene: 102247 Mouse
- Entrez Gene: 290843 Rat
- SwissProt: A3FPG8 Cow
- SwissProt: Q86UL3 Human
- SwissProt: Q8K2C8 Mouse
- SwissProt: Q0ZFS7 Rat
- Unigene: 355753 Human
see all -
Alternative names
- 1 acyl sn glycerol 3 phosphate acyltransferase zeta antibody
- 1 AGP acyltransferase 6 antibody
- 1 AGPAT 6 antibody
see all
Images
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Western blot - Anti-GPAT4 antibody (ab76707)Anti-GPAT4 antibody (ab76707) at 1 µg/ml + Human skeletal muscle tissue lysate - total protein (ab29330) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 minutes
The band observed at 49 kDa could potentially be a cleaved form of Human 1-acyl-sn-glycerol-3-phosphate acyltransferase zeta precursor (AGPAT6). The protein contains a 36 amino acid signal peptide, which when cleaved gives an expected molecular weight of 48 kDa.
Datasheets and documents
References (1)
ab76707 has been referenced in 1 publication.
- Sadh K et al. Feeding-fasting dependent recruitment of membrane microdomain proteins to lipid droplets purified from the liver. PLoS One 12:e0183022 (2017). PubMed: 28800633
Images
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Western blot - Anti-GPAT4 antibody (ab76707)Anti-GPAT4 antibody (ab76707) at 1 µg/ml + Human skeletal muscle tissue lysate - total protein (ab29330) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 minutes
The band observed at 49 kDa could potentially be a cleaved form of Human 1-acyl-sn-glycerol-3-phosphate acyltransferase zeta precursor (AGPAT6). The protein contains a 36 amino acid signal peptide, which when cleaved gives an expected molecular weight of 48 kDa.