Antibodies, Proteins, Kits and Reagents for Life Science

335 040 ₸ Product size

100 µl 335 040 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3754] to Peroxiredoxin 6
Suitable for: WB, IHC-P
Knockout validated
Reacts with: Mouse, Human

Product name

Anti-Peroxiredoxin 6 antibody [EPR3754]
See all Peroxiredoxin 6 primary antibodies
Description

Rabbit monoclonal [EPR3754] to Peroxiredoxin 6
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human Peroxiredoxin 6 aa 50-150. The exact sequence is proprietary.
Positive control
- HeLa, 293 and fetal liver lysates; Human brain tissue
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR3754
Isotype

IgG
Research areas

Western blot - Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)

All lanes : Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348) at 1/1000 dilution

Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : PRDX6 (Peroxiredoxin 6) knockout HAP1 whole cell lysate
Lane 3 : A431 whole cell lysate
Lane 4 : PC3 whole cell lysate

Lysates/proteins at 20 µg per lane.

Predicted band size: 25 kDa

Lanes 1 - 4: Merged signal (red and green). Green - ab133348 observed at 25 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab133348 was shown to specifically react with Peroxiredoxin 6 in wild-type HAP1 cells as signal was lost in PRDX6 (Peroxiredoxin 6) knockout cells. Wild-type and PRDX6 (Peroxiredoxin 6) knockout samples were subjected to SDS-PAGE. Ab133348 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)

Immunohistochemical analysis of paraffin embedded normal Human stomach tissue using ab133348 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)

All lanes : Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)

Lane 1 : HeLa lysate
Lane 2 : 293 lysate
Lane 3 : Fetal liver lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 25 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)

Immunohistochemical analysis of paraffin-embedded Human brain tissue labelling Peroxiredoxin 6 with ab133348 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)

Immunohistochemical analysis of paraffin embedded normal Human liver tissue using ab133348 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)

Immunohistochemical analysis of paraffin embedded normal Human placenta tissue using ab133348 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)

Immunohistochemical analysis of paraffin embedded normal Human testis tissue using ab133348 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)

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