Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HSPA12A antibody [EPR16763] - BSA and Azide free (ab236146)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16763] to HSPA12A - BSA and Azide free
  • Suitable for: IP, ICC/IF, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-HSPA12A antibody [EPR16763] - BSA and Azide free
    See all HSPA12A primary antibodies

  • Description

    Rabbit monoclonal [EPR16763] to HSPA12A - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, ICC/IF, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human kidney tissue.

  • General notes

    Ab236146 is the carrier-free version of ab200838. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab236146 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR16763

  • Isotype

    IgG

  • Research areas

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-HSPA12A antibody [EPR16763] - BSA and Azide free (ab236146)
    Immunocytochemistry/ Immunofluorescence - Anti-HSPA12A antibody [EPR16763] - BSA and Azide free (ab236146)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U87-MG (human glioblastoma) cells labeling HSPA12A with ab200838 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasmic staining on U87-MG cell line is observed. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200838).

  • Immunocytochemistry/ Immunofluorescence - Anti-HSPA12A antibody [EPR16763] - BSA and Azide free (ab236146)
    Immunocytochemistry/ Immunofluorescence - Anti-HSPA12A antibody [EPR16763] - BSA and Azide free (ab236146)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK293 (Human embryonic kidney) cells labeling HSPA12A with ab200838 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasmic staining on HEK293 cell line is observed. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200838).

  • Immunoprecipitation - Anti-HSPA12A antibody [EPR16763] - BSA and Azide free (ab236146)
    Immunoprecipitation - Anti-HSPA12A antibody [EPR16763] - BSA and Azide free (ab236146)

    HSPA12A was immunoprecipitated from 1mg of Human fetal brain whole cell lysate with ab200838 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab200838 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: Human fetal brain whole cell lysate 10 µg (Input). Lane 2: ab200838 IP in Human fetal brain whole cell lysate. Lane 3 : Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200838 in Human fetal brain whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

     

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200838).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA12A antibody [EPR16763] - BSA and Azide free (ab236146)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA12A antibody [EPR16763] - BSA and Azide free (ab236146)

    Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue labeling HSPA12A with ab200838 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on rat cerbral cortex tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200838).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA12A antibody [EPR16763] - BSA and Azide free (ab236146)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA12A antibody [EPR16763] - BSA and Azide free (ab236146)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling HSPA12A with ab200838 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on mouse cerbral cortex tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200838).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA12A antibody [EPR16763] - BSA and Azide free (ab236146)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA12A antibody [EPR16763] - BSA and Azide free (ab236146)

    Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling HSPA12A with ab200838 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on Human cerebral cortex tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200838).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA12A antibody [EPR16763] - BSA and Azide free (ab236146)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA12A antibody [EPR16763] - BSA and Azide free (ab236146)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling HSPA12A with ab200838 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on Human kidney tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200838).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

     

  • Anti-HSPA12A antibody [EPR16763] - BSA and Azide free (ab236146)
    Anti-HSPA12A antibody [EPR16763] - BSA and Azide free (ab236146)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-HSPA12A antibody [EPR16763] - BSA and Azide free (ab236146)

© 2021 Astana Biomed Group LLP. All rights reserved.