Antibodies, Proteins, Kits and Reagents for Life Science

247 259 ₸ Product size

100 µl 247 259 ₸

Order now and get it on Tuesday March 02, 2021

Anti-HSPA12A antibody [EPR16763] (ab200838)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR16763] to HSPA12A
Suitable for: WB, IHC-P, ICC/IF, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-HSPA12A antibody [EPR16763]
See all HSPA12A primary antibodies
Description

Rabbit monoclonal [EPR16763] to HSPA12A
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Mouse Rat Human
IP	Human
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HEK293 and U-87 MG cell lysates; Human fetal brain, Human fetal kidney, mouse spleen and rat heart lysates. IHC-P: Human cerebral cortex, Human kidney, mouse cerebral cortex and rat cerebral cortex tissues. ICC/IF: HEK293 cells. IP: Human fetal brain whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR16763
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-HSPA12A antibody [EPR16763] (ab200838)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U87-MG (human glioblastoma) cells labeling HSPA12A with ab200838 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasmic staining on U87-MG cell line is observed. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
Immunocytochemistry/ Immunofluorescence - Anti-HSPA12A antibody [EPR16763] (ab200838)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK293 (Human embryonic kidney) cells labeling HSPA12A with ab200838 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasmic staining on HEK293 cell line is observed. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
Western blot - Anti-HSPA12A antibody [EPR16763] (ab200838)

All lanes : Anti-HSPA12A antibody [EPR16763] (ab200838) at 1/1000 dilution

Lane 1 : HEK293 (Human embryonic kidney) cell lysate
Lane 2 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 75 kDa
Observed band size: 75 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-HSPA12A antibody [EPR16763] (ab200838)

Anti-HSPA12A antibody [EPR16763] (ab200838) at 1/10000 dilution + Human fetal brain lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 75 kDa
Observed band size: 75 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-HSPA12A antibody [EPR16763] (ab200838)

Anti-HSPA12A antibody [EPR16763] (ab200838) at 1/1000 dilution + Human fetal kidney lysate at 10 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 75 kDa
Observed band size: 75 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-HSPA12A antibody [EPR16763] (ab200838)

All lanes : Anti-HSPA12A antibody [EPR16763] (ab200838) at 1/1000 dilution

Lane 1 : Mouse spleen lysate
Lane 2 : Rat heart lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 75 kDa
Observed band size: 75 kDa

Exposure time: 1 minute

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA12A antibody [EPR16763] (ab200838)

Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling HSPA12A with ab200838 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on Human cerebral cortex tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA12A antibody [EPR16763] (ab200838)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling HSPA12A with ab200838 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on Human kidney tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA12A antibody [EPR16763] (ab200838)

Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling HSPA12A with ab200838 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on mouse cerbral cortex tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA12A antibody [EPR16763] (ab200838)

Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue labeling HSPA12A with ab200838 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on rat cerbral cortex tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-HSPA12A antibody [EPR16763] (ab200838)

HSPA12A was immunoprecipitated from 1mg of Human fetal brain whole cell lysate with ab200838 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab200838 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: Human fetal brain whole cell lysate 10 µg (Input). Lane 2: ab200838 IP in Human fetal brain whole cell lysate. Lane 3 : Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200838 in Human fetal brain whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
Anti-HSPA12A antibody [EPR16763] (ab200838)

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