Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Calretinin antibody [SP13] (ab16694)

Key features and details

  • Rabbit monoclonal [SP13] to Calretinin
  • Suitable for: IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Calretinin antibody [SP13]
    See all Calretinin primary antibodies

  • Description

    Rabbit monoclonal [SP13] to Calretinin

  • Host species

    Rabbit

  • Specificity

    Ab16694 recognises Calretinin.

  • Tested Applications & Species

    Application Species
    IHC-Fr
    Mouse
    Rat
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein within Mouse Calretinin. The exact sequence is proprietary.

  • Positive control

    • Human mesothelioma for Immunohistochemistry. IHC-Fr: Mouse and rat cerebrum tissue

  • General notes

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.50
    Preservative: 0.1% Sodium azide
    Constituents: Tissue culture supernatant, Tris buffered saline, 1% BSA
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    SP13

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Frozen sections) - Anti-Calretinin antibody [SP13] (ab16694)
    Immunohistochemistry (Frozen sections) - Anti-Calretinin antibody [SP13] (ab16694)

    Immunohistochemistry (Frozen) analysis of mouse cerebrum tissue section labeling Calretinin with purified ab16694 at 1/30 (9.6 µg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunohistochemistry (Frozen sections) - Anti-Calretinin antibody [SP13] (ab16694)
    Immunohistochemistry (Frozen sections) - Anti-Calretinin antibody [SP13] (ab16694)

    Immunohistochemistry (Frozen) analysis of rat cerebrum tissue section labeling Calretinin with purified ab16694 at 1/30 (9.6 µg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunohistochemistry (Frozen sections) - Anti-Calretinin antibody [SP13] (ab16694)
    Immunohistochemistry (Frozen sections) - Anti-Calretinin antibody [SP13] (ab16694)

    Immunohistochemistry (Frozen) analysis of rat cerebrum tissue section labeling Calretinin with purified ab16694 at 1/30 (9.6 µg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunohistochemistry (Frozen sections) - Anti-Calretinin antibody [SP13] (ab16694)
    Immunohistochemistry (Frozen sections) - Anti-Calretinin antibody [SP13] (ab16694)

    Immunohistochemistry (Frozen) analysis of mouse cerebrum tissue section labeling Calretinin with purified ab16694 at 1/30 (9.6 µg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody [SP13] (ab16694)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody [SP13] (ab16694)
    Ab16694 at a dilution of 1/100, staining Calretinin in formalin fixed paraffin embedded human mesothelioma tissue section by Immunohistochemistry.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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