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Signal Transduction Signaling Pathway Calcium Signaling Calcium Binding Proteins

Anti-Calretinin antibody [1F5H1] (ab204990)

Price and availability

274 732 ₸

Availability

Order now and get it on Wednesday March 10, 2021

Anti-Calretinin antibody [1F5H1] (ab204990)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [1F5H1] to Calretinin
  • Suitable for: Flow Cyt, IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-Calretinin antibody [1F5H1]
    See all Calretinin primary antibodies
  • Description

    Mouse monoclonal [1F5H1] to Calretinin
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant full length protein corresponding to Human Calretinin aa 1 to the C-terminus. (Purified from E.coli).
    Database link: P22676

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Human Calretinin (aa1-271) recombinant protein; Calretinin (aa1-271)-hIgGFc transfected HEK293 cell lysate; A549 cells; Human brain tissue.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    1F5H1
  • Isotype

    IgG1
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Calcium Signaling
    • Calcium Binding Proteins

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody [1F5H1] (ab204990)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody [1F5H1] (ab204990)

    Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling Calretinin with ab204990 at 1/200 dilution with DAB staining.

  • Western blot - Anti-Calretinin antibody [1F5H1] (ab204990)
    Western blot - Anti-Calretinin antibody [1F5H1] (ab204990)
    Anti-Calretinin antibody [1F5H1] (ab204990) at 1/500 dilution + Human Calretinin (aa1-271) recombinant protein

    Predicted band size: 31 kDa



    Expected MWt is 57.5 kDa.

  • Western blot - Anti-Calretinin antibody [1F5H1] (ab204990)
    Western blot - Anti-Calretinin antibody [1F5H1] (ab204990)
    All lanes : Anti-Calretinin antibody [1F5H1] (ab204990) at 1/500 dilution

    Lane 1 : HEK293 cell lysate
    Lane 2 : Calretinin (aa1-271)-hIgGFc transfected HEK293 cell lysate

    Predicted band size: 31 kDa

  • Flow Cytometry - Anti-Calretinin antibody [1F5H1] (ab204990)
    Flow Cytometry - Anti-Calretinin antibody [1F5H1] (ab204990)

    Flow cytometric analysis of A549 cells labeling Calretinin with ab204990 at 1/200 dilution (green), compared to a negative control (red).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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