Antibodies, Proteins, Kits and Reagents for Life Science

335 040 ₸ Product size

100 µl 335 040 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Calretinin antibody [EP1798] (ab92341)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP1798] to Calretinin
Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
Reacts with: Mouse, Rat, Human

Product name

Anti-Calretinin antibody [EP1798]
See all Calretinin primary antibodies
Description

Rabbit monoclonal [EP1798] to Calretinin
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human Calretinin aa 50-150. The exact sequence is proprietary.
Database link: P22676
Positive control
- Human fetal, Mouse and Rat brain lysates; Human brain and mesothelioma tissues.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EP1798
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody [EP1798] (ab92341)

ab92341 at 1/100 dilution staining Calretinin in Human mesothelioma by Immunohistochemistry, Paraffin-embedded tissue. The use of an HRP/AP polymerized antibody is recommended for a secondary antibody.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-Calretinin antibody [EP1798] (ab92341)

All lanes : Anti-Calretinin antibody [EP1798] (ab92341) at 1/1000 dilution

Lane 1 : Human fetal Brain lysate
Lane 2 : Mouse brain lysate
Lane 3 : Rat brain lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 29 kDa
Observed band size: 29 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody [EP1798] (ab92341)

ab92341 at 1/100 dilution staining Calretinin in Human brain by Immunohistochemistry, Paraffin-embedded tissue. The use of an HRP/AP polymerized antibody is recommended for a secondary antibody.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cytometry - Anti-Calretinin antibody [EP1798] (ab92341)

Overlay histogram showing SH-SY5Y cells stained with ab92341 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92341, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Immunocytochemistry/ Immunofluorescence - Anti-Calretinin antibody [EP1798] (ab92341)

Immunofluorecent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilsed SH-SY5Y (human neuroblastoma epithelial cell line) cells labelling Calretinin with ab92341 at 1/500 dilution, followed by AlexaFluor^®488 Goat anti-Rabbit (ab150077) secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic and nuclear staining on SH-SY5Y cell line is observed. Counterstained with DAPI.
Anti-Calretinin antibody [EP1798] (ab92341)

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