Anti-Calretinin antibody (ab244299)
Key features and details
- Rabbit polyclonal to Calretinin
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Calretinin antibody
See all Calretinin primary antibodies -
Description
Rabbit polyclonal to Calretinin -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow
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Immunogen
Recombinant fragment corresponding to Human Calretinin aa 100 to the C-terminus.
Database link: P22676 -
Positive control
- WB: Human cerebral cortex tissue lysate. Mouse cerebellum tissue lysate. NIH/3T3 cell lysate. ICC/IF: U-2 OS cells. IHC-P: Mouse substantia nigra, hippocampus, medulla, hypothalamus and hindbrain tissue. Human cerebral cortex, testis and liver tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody (ab244299)
Formalin-fixed, paraffin-embedded human stomach tissue stained for Calretinin with ab244299 at a 1/50 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody (ab244299)Formalin-fixed, paraffin-embedded mouse hippocampus tissue stained for Calretinin with ab244299 at a 1/50 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody (ab244299)Formalin-fixed, paraffin-embedded human cerebellum tissue stained for Calretinin with ab244299 at a 1/50 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-Calretinin antibody (ab244299)PFA fixed, Triton X-100 permeabilized U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling Calretinin using ab244299 at 4 µg/ml (green) in ICC/IF.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody (ab244299)Formalin-fixed, paraffin-embedded human testis tissue stained for Calretinin with ab244299 at a 1/50 dilution in immunohistochemical analysis.
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Western blot - Anti-Calretinin antibody (ab244299)All lanes : Anti-Calretinin antibody (ab244299) at 0.4 µg/ml
Lane 1 : NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate
Lane 2 : NBT-II (Rat cell line) cell lysate
Predicted band size: 32 kDa
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Western blot - Anti-Calretinin antibody (ab244299)All lanes : Anti-Calretinin antibody (ab244299) at 0.4 µg/ml
Lane 1 : U-2 OS (Human bone osteosarcoma epithelial cell line) transfected with control siRNA
Lane 2 : U-2 OS transfected with siRNA probe #1
Lane 3 : U-2 OS transfected with siRNA probe #2
Predicted band size: 32 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody (ab244299)Formalin-fixed, paraffin-embedded mouse hypothalamus tissue stained for Calretinin with ab244299 at a 1/50 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody (ab244299)Formalin-fixed, paraffin-embedded mouse medulla tissue stained for Calretinin with ab244299 at a 1/50 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody (ab244299)Formalin-fixed, paraffin-embedded mouse hindbrain tissue stained for Calretinin with ab244299 at a 1/50 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody (ab244299)Formalin-fixed, paraffin-embedded mouse substantia nigra tissue stained for Calretinin with ab244299 at a 1/50 dilution in immunohistochemical analysis.
