Antibodies, Proteins, Kits and Reagents for Life Science

274 732 ₸ Product size

100 µl 274 732 ₸ 10 µl 107 212 ₸

Order now and get it on Wednesday March 17, 2021

Anti-HPS1 antibody [EP8429(2)] (ab174301)

Key features and details

Rabbit monoclonal [EP8429(2)] to HPS1
Suitable for: WB, ICC/IF, Flow Cyt, IHC-P
Reacts with: Human
Isotype: IgG

Product name

Anti-HPS1 antibody [EP8429(2)]
See all HPS1 primary antibodies
Description

Rabbit monoclonal [EP8429(2)] to HPS1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human HPS1 aa 400-500 (Cysteine residue). The exact sequence is proprietary.
Database link: Q92902
Positive control
- A375, HeLa, HT-29 and K562 cell lysates; Human breast and muscle tissues; HeLa cells; K562 cells.
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EP8429(2)
Isotype

IgG
Research areas

Western blot - Anti-HPS1 antibody [EP8429(2)] (ab174301)

All lanes : Anti-HPS1 antibody [EP8429(2)] (ab174301) at 1/1000 dilution

Lane 1 : A375 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HT-29 cell lysate
Lane 4 : K562 cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 79 kDa
Immunocytochemistry/ Immunofluorescence - Anti-HPS1 antibody [EP8429(2)] (ab174301)

Immunofluorescent analysis of HeLa cells labeling HPS1 with ab174301 at 1/50 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HPS1 antibody [EP8429(2)] (ab174301)

Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling HPS1 with ab174301 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HPS1 antibody [EP8429(2)] (ab174301)

Immunohistochemical analysis of paraffin-embedded Human muscle tissue labeling HPS1 with ab174301 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cytometry - Anti-HPS1 antibody [EP8429(2)] (ab174301)

Flow cytometric analysis of permeabilized K562 cells with ab174301 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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