Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HPS1 antibody [EP8429(2)] (ab174301)

Key features and details

  • Rabbit monoclonal [EP8429(2)] to HPS1
  • Suitable for: WB, ICC/IF, Flow Cyt, IHC-P
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-HPS1 antibody [EP8429(2)]
    See all HPS1 primary antibodies

  • Description

    Rabbit monoclonal [EP8429(2)] to HPS1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human HPS1 aa 400-500 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q92902

  • Positive control

    • A375, HeLa, HT-29 and K562 cell lysates; Human breast and muscle tissues; HeLa cells; K562 cells.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EP8429(2)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-HPS1 antibody [EP8429(2)] (ab174301)
    Western blot - Anti-HPS1 antibody [EP8429(2)] (ab174301)
    All lanes : Anti-HPS1 antibody [EP8429(2)] (ab174301) at 1/1000 dilution

    Lane 1 : A375 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HT-29 cell lysate
    Lane 4 : K562 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 79 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-HPS1 antibody [EP8429(2)] (ab174301)
    Immunocytochemistry/ Immunofluorescence - Anti-HPS1 antibody [EP8429(2)] (ab174301)

    Immunofluorescent analysis of HeLa cells labeling HPS1 with ab174301 at 1/50 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HPS1 antibody [EP8429(2)] (ab174301)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HPS1 antibody [EP8429(2)] (ab174301)

    Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling HPS1 with ab174301 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HPS1 antibody [EP8429(2)] (ab174301)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HPS1 antibody [EP8429(2)] (ab174301)

    Immunohistochemical analysis of paraffin-embedded Human muscle tissue labeling HPS1 with ab174301 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-HPS1 antibody [EP8429(2)] (ab174301)
    Flow Cytometry - Anti-HPS1 antibody [EP8429(2)] (ab174301)

    Flow cytometric analysis of permeabilized K562 cells with ab174301 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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