Antibodies, Proteins, Kits and Reagents for Life Science

Anti-GOLPH2 antibody [OTI6C9] (ab119800)

Key features and details

Mouse monoclonal [OTI6C9] to GOLPH2
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
Reacts with: Mouse, Dog, Human, African green monkey
Isotype: IgG1

Product name

Anti-GOLPH2 antibody [OTI6C9]
See all GOLPH2 primary antibodies
Description

Mouse monoclonal [OTI6C9] to GOLPH2
Host species

Mouse

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	African green monkey
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant full length protein corresponding to Human GOLPH2 aa 1 to the C-terminus. Produced in HEK-293T cells (NP_808800).
Database link: Q8NBJ4

Run BLAST with Run BLAST with
Positive control
- WB: HEK-293T cell lysate transfected with pCMV-ENTRY GOLPH2 cDNA; HeLa, A549 and COS-7 cell extracts. IHC-P: Human ovary adenocarcinoma, thyroid carcinoma, endometrium, endometrium adenocarcinoma, prostate and bladder carcinoma tissues. ICC/IF: COS-7 cells transiently transfected by pCMV6-ENTRY GOLPH2.
General notes

The clone number has been updated from 6C9 to OTI6C9, both clone numbers name the same clone.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Concentration information loading...
Purity

Affinity purified
Purification notes

Purified from cell culture supernatant by affinity chromatography.
Clonality

Monoclonal
Clone number

OTI6C9
Isotype

IgG1
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLPH2 antibody [OTI6C9] (ab119800)

Paraffin-embedded human ovary adenocarcinoma tissue stained for GOLPH2 using ab119800 at 1/150 dilution in immunohistochemical analysis.
Flow Cytometry - Anti-GOLPH2 antibody [OTI6C9] (ab119800)

Overlay histogram showing HeLa cells stained with ab119800 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab119800, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Western blot - Anti-GOLPH2 antibody [OTI6C9] (ab119800)

All lanes : Anti-GOLPH2 antibody [OTI6C9] (ab119800) at 1/500 dilution

Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY GOLPH2

Lysates/proteins at 5 µg per lane.

Predicted band size: 45 kDa
Western blot - Anti-GOLPH2 antibody [OTI6C9] (ab119800)

All lanes : Anti-GOLPH2 antibody [OTI6C9] (ab119800) at 1/500 dilution

Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) cell extract
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lane 3 : SVT2 cell extract
Lane 4 : A549 (human lung carcinoma cell line) cell extract
Lane 5 : COS-7 (african green monkey kidney fibroblast-like cell line) cell extract
Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) cell extract
Lane 7 : MDCK (canine kidney cell line) cell extract
Lane 8 : PC-12 (rat adrenal gland pheochromocytoma cell line) cell extract
Lane 9 : MCF7 (human breast adenocarcinoma cell line) cell extract

Lysates/proteins at 35 µg per lane.

Predicted band size: 45 kDa

HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC200086)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLPH2 antibody [OTI6C9] (ab119800)

Paraffin-embedded human thyroid carcinoma tissue stained for GOLPH2 using ab119800 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLPH2 antibody [OTI6C9] (ab119800)

Paraffin-embedded human endometrium tissue stained for GOLPH2 using ab119800 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLPH2 antibody [OTI6C9] (ab119800)

Paraffin-embedded human endometrium adenocarcinoma tissue stained for GOLPH2 using ab119800 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLPH2 antibody [OTI6C9] (ab119800)

Paraffin-embedded human prostate tissue stained for GOLPH2 using ab119800 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLPH2 antibody [OTI6C9] (ab119800)

Paraffin-embedded human bladder carcinoma tissue stained for GOLPH2 using ab119800 at 1/150 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-GOLPH2 antibody [OTI6C9] (ab119800)

COS-7 (african green monkey kidney fibroblast-like cell line) cells transiently transfected by pCMV6-ENTRY GOLPH2 stained for GOLPH2 (green) using ab119800 at 1/100 dilution in ICC/IF.

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