Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GOLPH2 antibody [OTI6C9] (ab119800)

Key features and details

  • Mouse monoclonal [OTI6C9] to GOLPH2
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Dog, Human, African green monkey
  • Isotype: IgG1

Overview

  • Product name

    Anti-GOLPH2 antibody [OTI6C9]
    See all GOLPH2 primary antibodies

  • Description

    Mouse monoclonal [OTI6C9] to GOLPH2

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    African green monkey
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein corresponding to Human GOLPH2 aa 1 to the C-terminus. Produced in HEK-293T cells (NP_808800).
    Database link: Q8NBJ4

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: HEK-293T cell lysate transfected with pCMV-ENTRY GOLPH2 cDNA; HeLa, A549 and COS-7 cell extracts. IHC-P: Human ovary adenocarcinoma, thyroid carcinoma, endometrium, endometrium adenocarcinoma, prostate and bladder carcinoma tissues. ICC/IF: COS-7 cells transiently transfected by pCMV6-ENTRY GOLPH2.

  • General notes

    The clone number has been updated from 6C9 to OTI6C9, both clone numbers name the same clone.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 1% BSA, 50% Glycerol
  • Concentration information loading...

  • Purity

    Affinity purified

  • Purification notes

    Purified from cell culture supernatant by affinity chromatography.

  • Clonality

    Monoclonal

  • Clone number

    OTI6C9

  • Isotype

    IgG1

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLPH2 antibody [OTI6C9] (ab119800)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLPH2 antibody [OTI6C9] (ab119800)

    Paraffin-embedded human ovary adenocarcinoma tissue stained for GOLPH2 using ab119800 at 1/150 dilution in immunohistochemical analysis.

  • Flow Cytometry - Anti-GOLPH2 antibody [OTI6C9] (ab119800)
    Flow Cytometry - Anti-GOLPH2 antibody [OTI6C9] (ab119800)
    Overlay histogram showing HeLa cells stained with ab119800 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab119800, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Western blot - Anti-GOLPH2 antibody [OTI6C9] (ab119800)
    Western blot - Anti-GOLPH2 antibody [OTI6C9] (ab119800)
    All lanes : Anti-GOLPH2 antibody [OTI6C9] (ab119800) at 1/500 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY GOLPH2

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 45 kDa

  • Western blot - Anti-GOLPH2 antibody [OTI6C9] (ab119800)
    Western blot - Anti-GOLPH2 antibody [OTI6C9] (ab119800)
    All lanes : Anti-GOLPH2 antibody [OTI6C9] (ab119800) at 1/500 dilution

    Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) cell extract
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
    Lane 3 : SVT2 cell extract
    Lane 4 : A549 (human lung carcinoma cell line) cell extract
    Lane 5 : COS-7 (african green monkey kidney fibroblast-like cell line) cell extract
    Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) cell extract
    Lane 7 : MDCK (canine kidney cell line) cell extract
    Lane 8 : PC-12 (rat adrenal gland pheochromocytoma cell line) cell extract
    Lane 9 : MCF7 (human breast adenocarcinoma cell line) cell extract

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 45 kDa



    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC200086)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLPH2 antibody [OTI6C9] (ab119800)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLPH2 antibody [OTI6C9] (ab119800)

    Paraffin-embedded human thyroid carcinoma tissue stained for GOLPH2 using ab119800 at 1/150 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLPH2 antibody [OTI6C9] (ab119800)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLPH2 antibody [OTI6C9] (ab119800)

    Paraffin-embedded human endometrium tissue stained for GOLPH2 using ab119800 at 1/150 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLPH2 antibody [OTI6C9] (ab119800)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLPH2 antibody [OTI6C9] (ab119800)

    Paraffin-embedded human endometrium adenocarcinoma tissue stained for GOLPH2 using ab119800 at 1/150 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLPH2 antibody [OTI6C9] (ab119800)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLPH2 antibody [OTI6C9] (ab119800)

    Paraffin-embedded human prostate tissue stained for GOLPH2 using ab119800 at 1/150 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLPH2 antibody [OTI6C9] (ab119800)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLPH2 antibody [OTI6C9] (ab119800)

    Paraffin-embedded human bladder carcinoma tissue stained for GOLPH2 using ab119800 at 1/150 dilution in immunohistochemical analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-GOLPH2 antibody [OTI6C9] (ab119800)
    Immunocytochemistry/ Immunofluorescence - Anti-GOLPH2 antibody [OTI6C9] (ab119800)

    COS-7 (african green monkey kidney fibroblast-like cell line) cells transiently transfected by pCMV6-ENTRY GOLPH2 stained for GOLPH2 (green) using ab119800 at 1/100 dilution in ICC/IF.

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