Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GOLPH2 antibody [EPR3606] (ab109628)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3606] to GOLPH2
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-GOLPH2 antibody [EPR3606]
    See all GOLPH2 primary antibodies

  • Description

    Rabbit monoclonal [EPR3606] to GOLPH2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC: Human colon and small intestine tissue; WB: LNCaP, Huh7, MDA-MB-231, HepG2, and SH-SY5Y whole cell lysates.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-GOLPH2 antibody [EPR3606] (ab109628)
    Western blot - Anti-GOLPH2 antibody [EPR3606] (ab109628)
    All lanes : Anti-GOLPH2 antibody [EPR3606] (ab109628) at 1/1000 dilution (Purified)

    Lane 1 : Huh7 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
    Lane 2 : MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysate
    Lane 3 : LNCaP (Human prostate carcinoma epithelial cell) whole cell lysate
    Lane 4 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 45 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?



    The molecular weight observed is consistent with what has been described in PMID:18953438 and PMID: 10831838.
    The expression level of GOLPH2 in Huh7 and HepG2 cells is consistent with what has been described in PMID: 27569582.

    Blocking/Diluting buffer: 5% NFDM/TBST

    Loading Control: Rabbit monoclonal [EPR16891] to GAPDH (ab181602)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLPH2 antibody [EPR3606] (ab109628)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLPH2 antibody [EPR3606] (ab109628)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labeling GOLPH2 with purified ab109628 at 1/3000 dilution (0.19 µg/mL). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Western blot - Anti-GOLPH2 antibody [EPR3606] (ab109628)
    Western blot - Anti-GOLPH2 antibody [EPR3606] (ab109628)
    All lanes : Anti-GOLPH2 antibody [EPR3606] (ab109628) at 1/1000 dilution (unpurified)

    Lane 1 : LnCaP cell lysate
    Lane 2 : SH-SY5Y cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled Goat anti-Rabbit at 1/2000 dilution

    Predicted band size: 45 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLPH2 antibody [EPR3606] (ab109628)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLPH2 antibody [EPR3606] (ab109628)

    Immunohistochemical analysis of GOLPH2 in paraffin-embedded Human colon tissue using ab109628, unpurified, at 1/500 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLPH2 antibody [EPR3606] (ab109628)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLPH2 antibody [EPR3606] (ab109628) This image is courtesy of an anonymous Abreview

    ab109628, unpurified, staining GOLPH2 in Human small intestine by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

    Tissue was fixed with paraformaldehyde and blocked with 5% milk for 30 min at 37°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/1000 in diluent) for 1 hour at 37°C. An undiluted HRP-conjugated goat anti-rabbit polyclonal IgG was used as the secondary antibody.

    See Abreview

  • Anti-GOLPH2 antibody [EPR3606] (ab109628)
    Anti-GOLPH2 antibody [EPR3606] (ab109628)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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