Antibodies, Proteins, Kits and Reagents for Life Science

274 732 ₸ Product size

100 µl 274 732 ₸

Order now and get it on Tuesday March 02, 2021

Anti-GOLPH2 antibody [EPR3606] (ab109628)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3606] to GOLPH2
Suitable for: WB, IHC-P
Reacts with: Human

Product name

Anti-GOLPH2 antibody [EPR3606]
See all GOLPH2 primary antibodies
Description

Rabbit monoclonal [EPR3606] to GOLPH2
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC: Human colon and small intestine tissue; WB: LNCaP, Huh7, MDA-MB-231, HepG2, and SH-SY5Y whole cell lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR3606
Isotype

IgG
Research areas

Western blot - Anti-GOLPH2 antibody [EPR3606] (ab109628)

All lanes : Anti-GOLPH2 antibody [EPR3606] (ab109628) at 1/1000 dilution (Purified)

Lane 1 : Huh7 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 2 : MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : LNCaP (Human prostate carcinoma epithelial cell) whole cell lysate
Lane 4 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 45 kDa
Observed band size: 75 kDa
why is the actual band size different from the predicted?

The molecular weight observed is consistent with what has been described in PMID:18953438 and PMID: 10831838.
The expression level of GOLPH2 in Huh7 and HepG2 cells is consistent with what has been described in PMID: 27569582.

Blocking/Diluting buffer: 5% NFDM/TBST

Loading Control: Rabbit monoclonal [EPR16891] to GAPDH (ab181602)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLPH2 antibody [EPR3606] (ab109628)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labeling GOLPH2 with purified ab109628 at 1/3000 dilution (0.19 µg/mL). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Western blot - Anti-GOLPH2 antibody [EPR3606] (ab109628)

All lanes : Anti-GOLPH2 antibody [EPR3606] (ab109628) at 1/1000 dilution (unpurified)

Lane 1 : LnCaP cell lysate
Lane 2 : SH-SY5Y cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled Goat anti-Rabbit at 1/2000 dilution

Predicted band size: 45 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLPH2 antibody [EPR3606] (ab109628)

Immunohistochemical analysis of GOLPH2 in paraffin-embedded Human colon tissue using ab109628, unpurified, at 1/500 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLPH2 antibody [EPR3606] (ab109628) This image is courtesy of an anonymous Abreview

ab109628, unpurified, staining GOLPH2 in Human small intestine by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Tissue was fixed with paraformaldehyde and blocked with 5% milk for 30 min at 37°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/1000 in diluent) for 1 hour at 37°C. An undiluted HRP-conjugated goat anti-rabbit polyclonal IgG was used as the secondary antibody.
See Abreview
Anti-GOLPH2 antibody [EPR3606] (ab109628)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com