Antibodies, Proteins, Kits and Reagents for Life Science

335 040 ₸ Product size

100 µl 335 040 ₸

Order now and get it on Tuesday March 09, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4901] to NEK7
Suitable for: WB, Flow Cyt
Knockout validated
Reacts with: Human

Product name

Anti-NEK7 antibody [EPR4901]
See all NEK7 primary antibodies
Description

Rabbit monoclonal [EPR4901] to NEK7
Host species

Rabbit
Tested Applications & Species

Application Species
Flow Cyt
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Human brain, HeLa, A549, HepG2, fetal liver, and Jurkat lysates; Jurkat cells
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
Flow Cyt	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR4901
Isotype

IgG
Research areas

Western blot - Anti-NEK7 antibody [EPR4901] (ab109433)

Lane 1: Wild-type HAP1 cell lysate (40 µg)
Lane 2: NEK7 knockout HAP1 cell lysate (40 µg)
Lane 3: A549 cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab109433 observed at 35 kDa. Red - loading control, ab18058, observed at 124 kDa.

ab109433 was shown to specifically react with NEK7 when NEK7 knockout samples were used. Wild-type and NEK7 knockout samples were subjected to SDS-PAGE. Ab109433 and ab18058 (loading control to Vinculin) were diluted at 1/1000 and 1/10000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD)preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-NEK7 antibody [EPR4901] (ab109433)

All lanes : Anti-NEK7 antibody [EPR4901] (ab109433) at 1/10000 dilution

Lane 1 : Human brain lysate
Lane 2 : HeLa cell lysate
Lane 3 : A549 cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : Fetal liver lysate
Lane 6 : Jurkat cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 35 kDa
Flow Cytometry - Anti-NEK7 antibody [EPR4901] (ab109433)

Flow cytometric analysis of NEK7 in permeabilized Jurkat cells using ab109433 at 1/100 dilution (red) or a rabbit IgG (negative) (green).
Anti-NEK7 antibody [EPR4901] (ab109433)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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