Antibodies, Proteins, Kits and Reagents for Life Science

257 980 ₸ Product size

100 µl 257 980 ₸

Order now and get it on Tuesday March 09, 2021

Anti-GKN2 antibody [EPR15377(B)] - C-terminal (ab188866)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR15377(B)] to GKN2 - C-terminal
Suitable for: IHC-P, IP, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-GKN2 antibody [EPR15377(B)] - C-terminal
See all GKN2 primary antibodies
Description

Rabbit monoclonal [EPR15377(B)] to GKN2 - C-terminal
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Rat Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Human, mouse and rat stomach lysates; Mouse and rat stomach tissues.
General notes
This product was previously labelled as Gastrokine 2.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR15377(B)
Isotype

IgG
Research areas

Western blot - Anti-GKN2 antibody [EPR15377(B)] - C-terminal (ab188866)

All lanes : Anti-GKN2 antibody [EPR15377(B)] - C-terminal (ab188866) at 1/5000 dilution

Lane 1 : Human stomach lysate
Lane 2 : Mouse stomach lysate
Lane 3 : Rat stomach lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 20 kDa
Observed band size: 18 kDa
why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GKN2 antibody [EPR15377(B)] - C-terminal (ab188866)

Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling GKN2 with ab188866 at 1/4000 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GKN2 antibody [EPR15377(B)] - C-terminal (ab188866)

Immunohistochemical analysis of paraffin-embedded rat stomach tissue labeling GKN2 with ab188866 at 1/4,000 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-GKN2 antibody [EPR15377(B)] - C-terminal (ab188866)

Western blot analysis of GKN2 in Human stomach lysate immunoprecipitated using ab188866 at 1/50 dilution.

Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
Anti-GKN2 antibody [EPR15377(B)] - C-terminal (ab188866)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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