Anti-Abi-1 antibody (ab72318)
Key features and details
- Rabbit polyclonal to Abi-1
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Abi-1 antibody
See all Abi-1 primary antibodies -
Description
Rabbit polyclonal to Abi-1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus
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Immunogen
Synthetic peptide within Human Abi-1 aa 325-375. The exact sequence is proprietary.
Database link: NP_005461.2 -
Positive control
- Whole cell lysates from HeLa and 293T cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Abi-1 antibody (ab72318)All lanes : Anti-Abi-1 antibody (ab72318) at 0.04 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Predicted band size: 55 kDa
Observed band size: 55,60 kDa why is the actual band size different from the predicted?
Additional bands at: 28 kDa, 90 kDa. We are unsure as to the identity of these extra bands.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Abi-1 antibody (ab72318)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling Abi-1 with ab72318 at 1/200 (1µg/ml). Detection: DAB.
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Immunoprecipitation - Anti-Abi-1 antibody (ab72318)Detection of Human Abi-1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Lane 1: ab72317 used for IP at 3 µg/mg lysate. Lane 2: Abi-1 immunoprecipitated with an other antibody. Lane 3: ab72318 at 3 µg/mg lysate used for IP of Abi-1, which recognize downstream epitopes. Lane 4: control Ig IP For blotting immunoprecipitated Abi-1, ab72318 was used at 1 µg/ml.
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Immunoprecipitation - Anti-Abi-1 antibody (ab72318)Detection of Abi-1 by Immunoprecipitation from Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab72318 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent WB detection. Lane 2 represents control IgG IP.
