Human GST3 / GST pi ELISA Kit (ab277423)
Key features and details
- Sensitivity: 0.41 ng/ml
- Range: 0.41 ng/ml - 100 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human GST3 / GST pi ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.41 ng/ml -
Range
0.41 ng/ml - 100 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 75.11 71% - 79% Plasma 80.8 70% - 97% Cell culture media 86.52 79% - 90% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human GST3 / GST pi ELISA Kit (ab277423) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human GST3 / GST pi in serum (human GST3 / GST pi concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants.
This assay employs an antibody specific for Human GST3 / GST pi coated on a 96-well plate. Standards and samples are pipetted into the wells and Human GST3 / GST pi present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human GST3 / GST pi antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human GST3 / GST pi bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent 1 x 15ml Biotinylated Anti-Human GST3 / GST pi Antibody 2 vials Human GST3 / GST pi standard protein (Lyophilized) 2 vials Anti-Human GST3 GST pi coated Microplate 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. -
Sequence similarities
Belongs to the GST superfamily. Pi family.
Contains 1 GST C-terminal domain.
Contains 1 GST N-terminal domain. - Information by UniProt
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Alternative names
- Deafness
- Deafness X-linked 7
- DFN7
see all -
Database links
- Entrez Gene: 2950 Human
- Omim: 134660 Human
- SwissProt: P09211 Human
- Unigene: 523836 Human
Images
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Example data
These standard curves are for demonstration only. A standard curve must be run with each assay.