Anti-Geminin antibody (ab12147)
Key features and details
- Rabbit polyclonal to Geminin
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Geminin antibody
See all Geminin primary antibodies -
Description
Rabbit polyclonal to Geminin -
Host species
Rabbit -
Specificity
This antibody detects a band representing Geminin by Western blot at 33kD on 293T cell lysate, this band can be blocked by the immunising peptide. It also detects a band at about 45kD that is not blocked by the immunising peptide and is thought to be non-specific. The immunogen shares low homology with Mouse Geminin, however some customers have reported positive results when testing on mouse. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human Geminin aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab12408) -
Positive control
- This antibody gave a positive signal in Hek293 whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab12147 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanApplication Abreviews Notes WB (4) 1/500. Detects a band of approximately 33 kDa (predicted molecular weight: 23.5 kDa).Notes WB
1/500. Detects a band of approximately 33 kDa (predicted molecular weight: 23.5 kDa).Target
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Function
Inhibits DNA replication by preventing the incorporation of MCM complex into prereplication complex (pre-RC). It is degraded during the mitotic phase of the cell cycle. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle. -
Sequence similarities
Belongs to the geminin family. -
Developmental stage
Absent during G1 phase, accumulates during S, G2, and M phases, and disappears at the time of the metaphase-anaphase transition. - Information by UniProt
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Database links
- Entrez Gene: 51053 Human
- Omim: 602842 Human
- SwissProt: O75496 Human
- Unigene: 234896 Human
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Alternative names
- DNA replication inhibitor antibody
- Gem antibody
- GEMI_HUMAN antibody
see all
Images
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Western blot - Anti-Geminin antibody (ab12147)
Western blot using ab12147 at 1/500.
Lane 1: 293 lysate
The lower band represents Geminin (33 kDa - predicted molecular weight 23 kDa), and is blocked by the immunising peptide, the upper band is likely to be non-specific as it is not blocked by the peptide.
Lane 2: 293 lysate with blocking peptide ab12408.
Lysate at 5µ
The lower band represents Geminin (33kD - predicted molecular weight 23kd), and is blocked by the immunising peptide, the upper band is likely to be non-specific as it is not blocked by the peptide. -
Western blot - Anti-Geminin antibody (ab12147)Lane 1 : Anti-Geminin antibody (ab12147) at 1 µg/ml
Lane 2 : Anti-6X His tag® antibody (ab9108) at 1 µg/ml
All lanes : Recombinant Human Geminin protein (ab40570)
Lysates/proteins at 0.1 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 23.5 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted? -
Western blot - Anti-Geminin antibody (ab12147)This image is a courtesy of Anonymous AbreviewAnti-Geminin antibody (ab12147) at 1/500 dilution + Cell lysate prepared from human HeLa nuclear extract at 50 µg
Secondary
HRP-conjugated goat poyclonal to rabbit IgG at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 23.5 kDa
Observed band size: 33 kDa why is the actual band size different from the predicted?
Additional bands at: 95 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 minutes
Protocols
Datasheets and documents
References (9)
ab12147 has been referenced in 9 publications.
- Wasserman D et al. Cell cycle oscillators underlying orderly proteolysis of E2F8. Mol Biol Cell 31:725-740 (2020). PubMed: 31995441
- Ryan D et al. Targeting AXL and RAGE to prevent geminin overexpression-induced triple-negative breast cancer metastasis. Sci Rep 9:19150 (2019). PubMed: 31844158
- Tan J & Martin SE Validation of Synthetic CRISPR Reagents as a Tool for Arrayed Functional Genomic Screening. PLoS One 11:e0168968 (2016). PubMed: 28030641
- Zhang S et al. lncRNA Up-Regulated in Nonmuscle Invasive Bladder Cancer Facilitates Tumor Growth and Acts as a Negative Prognostic Factor of Recurrence. J Urol 196:1270-8 (2016). PubMed: 27267320
- Liu P et al. Cell-cycle-regulated activation of Akt kinase by phosphorylation at its carboxyl terminus. Nature 508:541-5 (2014). Mouse . PubMed: 24670654
- Maya-Mendoza A et al. Immortalised breast epithelia survive prolonged DNA replication stress and return to cycle from a senescent-like state. Cell Death Dis 5:e1351 (2014). WB, ICC/IF ; Human . PubMed: 25058425
- Cordero-Espinoza L & Hagen T Increased concentrations of fructose 2,6-bisphosphate contribute to the Warburg effect in phosphatase and tensin homolog (PTEN)-deficient cells. J Biol Chem 288:36020-8 (2013). WB ; Mouse . PubMed: 24169697
- Zhang AT et al. Dynamic interaction of Y RNAs with chromatin and initiation proteins during human DNA replication. J Cell Sci 124:2058-69 (2011). PubMed: 21610089
- Hochegger H et al. An essential role for Cdk1 in S phase control is revealed via chemical genetics in vertebrate cells. J Cell Biol 178:257-68 (2007). WB ; Human . PubMed: 17635936
Images
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Western blot - Anti-Geminin antibody (ab12147)
Western blot using ab12147 at 1/500.
Lane 1: 293 lysate
The lower band represents Geminin (33 kDa - predicted molecular weight 23 kDa), and is blocked by the immunising peptide, the upper band is likely to be non-specific as it is not blocked by the peptide.
Lane 2: 293 lysate with blocking peptide ab12408.
Lysate at 5µ
The lower band represents Geminin (33kD - predicted molecular weight 23kd), and is blocked by the immunising peptide, the upper band is likely to be non-specific as it is not blocked by the peptide. -
Western blot - Anti-Geminin antibody (ab12147)Lane 1 : Anti-Geminin antibody (ab12147) at 1 µg/ml
Lane 2 : Anti-6X His tag® antibody (ab9108) at 1 µg/ml
All lanes : Recombinant Human Geminin protein (ab40570)
Lysates/proteins at 0.1 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 23.5 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
-
Western blot - Anti-Geminin antibody (ab12147) This image is a courtesy of Anonymous AbreviewAnti-Geminin antibody (ab12147) at 1/500 dilution + Cell lysate prepared from human HeLa nuclear extract at 50 µg
Secondary
HRP-conjugated goat poyclonal to rabbit IgG at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 23.5 kDa
Observed band size: 33 kDa why is the actual band size different from the predicted?
Additional bands at: 95 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 minutes
