Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Geminin antibody [EPR14637] - BSA and Azide free (ab238988)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14637] to Geminin - BSA and Azide free
  • Suitable for: IHC-P, WB, ICC/IF
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Geminin antibody [EPR14637] - BSA and Azide free
    See all Geminin primary antibodies

  • Description

    Rabbit monoclonal [EPR14637] to Geminin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human tonsil tissue.

  • General notes

    Ab238988 is the carrier-free version of ab195047. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab238988 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Geminin antibody [EPR14637] - BSA and Azide free (ab238988)
    Western blot - Anti-Geminin antibody [EPR14637] - BSA and Azide free (ab238988)
    All lanes : Anti-Geminin antibody [EPR14637] (ab195047) at 1/500 dilution

    Lane 1 : Wild-type HeLa lysate
    Lane 2 : Geminin DNA Replication Inhibitor knockout HeLa lysate
    Lane 3 : HepG2 lysate
    Lane 4 : U-87 MG lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 24 kDa



    This data was developed using the same antibody clone in a different buffer formulation (ab195047).

    Lanes 1-4: Merged signal (red and green). Green - ab195047 observed at 32 kDa. Red - loading control ab7291 observed at 50 kDa.

     ab195047 Anti-Geminin antibody [EPR14637] was shown to specifically react with Geminin DNA Replication Inhibitor in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264972 (knockout cell lysate ab257450) was used. Wild-type and Geminin DNA Replication Inhibitor knockout samples were subjected to SDS-PAGE. ab195047 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 500 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

     

  • Immunocytochemistry/ Immunofluorescence - Anti-Geminin antibody [EPR14637] - BSA and Azide free (ab238988)
    Immunocytochemistry/ Immunofluorescence - Anti-Geminin antibody [EPR14637] - BSA and Azide free (ab238988) This image is courtesy of an Abreview submitted by Kirk McManus.

    ab195047 staining Geminin in the HeLa cell line from Human cervix by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 Samples were incubated with primary antibody (1/50 in PBS ) for 1 hour at 22°C. Ab150081 (1/200) was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab195047).

  • Immunocytochemistry/ Immunofluorescence - Anti-Geminin antibody [EPR14637] - BSA and Azide free (ab238988)
    Immunocytochemistry/ Immunofluorescence - Anti-Geminin antibody [EPR14637] - BSA and Azide free (ab238988)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF-7 (Human breast adenocarcinoma cell line) cells labeling Geminin with ab195047 at 1/100 followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500(green). Confocal image showing nuclear staining on MCF-7 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500  (red).

    The negative controls are as follows:-
    -ve control 1 - ab195047 at 1/100 followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500.
    -ve control 2. - ab7291 (anti-Tubulin mouse mAb) at 1/1000 followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab195047).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Geminin antibody [EPR14637] - BSA and Azide free (ab238988)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Geminin antibody [EPR14637] - BSA and Azide free (ab238988)

    Immunohistochemical analysis of paraffin-embedded Human triple-negative breast cancer tissue labeling Geminin with ab195047 at 1/500 followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. Nucleus staining on Human triple-negative breast cancer tissue is observed. Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary antibody and ab97051 at 1/500 as secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab195047).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Geminin antibody [EPR14637] - BSA and Azide free (ab238988)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Geminin antibody [EPR14637] - BSA and Azide free (ab238988)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Geminin with ab195047 at 1/500 followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. Nucleus staining on Human tonsil tissue is observed. Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody and ab97051 at 1/500 as secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab195047).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-Geminin antibody [EPR14637] - BSA and Azide free (ab238988)
    Anti-Geminin antibody [EPR14637] - BSA and Azide free (ab238988)

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