Anti-Geminin antibody (ab229009)
Key features and details
- Rabbit polyclonal to Geminin
- Suitable for: WB, ICC/IF, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Geminin antibody
See all Geminin primary antibodies -
Description
Rabbit polyclonal to Geminin -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rhesus monkey
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Immunogen
Recombinant full length protein corresponding to Human Geminin aa 1 to the C-terminus.
Database link: O75496 -
Positive control
- WB: HCT 116 whole cell lysate. IP: HCT 116 whole cell extract. IHC-P: Human breast cancer tissue. ICC/IF: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Geminin antibody (ab229009)Anti-Geminin antibody (ab229009) at 1/1000 dilution + HCT 116 (Human colorectal carcinoma cell line) whole cell lysate at 30 µg
Predicted band size: 24 kDa12% SDS PAGE
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Immunoprecipitation - Anti-Geminin antibody (ab229009)Geminin was immunoprecipitated from HCT 116 (human colorectal carcinoma cell line) whole cell extract with 5 μg of ab229009. Western blot was performed from the immunoprecipitate using ab229009. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: Control IgG instead of ab229009 in HCT 116 whole cell etract.
Lane 2: ab229009 IP in HCT116 whole cell extract. -
Immunocytochemistry/ Immunofluorescence - Anti-Geminin antibody (ab229009)Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Geminin (green) using ab229009 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Geminin antibody (ab229009)Paraffin-embedded human breast cancer tissue stained for Geminin using ab229009 at 1/250 dilution in immunohistochemical analysis.
