Anti-Geminin antibody (ab104306)
Key features and details
- Mouse monoclonal to Geminin
- Suitable for: IHC-P, ICC/IF, WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Geminin antibody
See all Geminin primary antibodies -
Description
Mouse monoclonal to Geminin -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanWB Human
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Immunogen
Recombinant fragment, corresponding to amino acids 110-209 of Human Geminin (AAH05185) with a proprietary tag.
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Positive control
- Partial tagged recombinant Geminin; Geminin transfected 293T cell lysate; Human lymph node; Human placenta; HeLa cells.
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General notes
This product was changed from ascites to tissue culture supernatant on 22 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Constituent: 2.68% PBS -
Concentration information loading... -
Purity
Tissue culture supernatant -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-Geminin antibody (ab104306)Anti-Geminin antibody (ab104306) at 5 µg/ml + Immunogen at 0.2 µg
Predicted band size: 24 kDaThe tagged immunogen has a predicted MWt of 37 kDa.
This image was generated using the ascites version of the product.
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Western blot - Anti-Geminin antibody (ab104306)All lanes : Anti-Geminin antibody (ab104306) at 5 µg/ml
Lane 1 : Geminin transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 24 kDaThis image was generated using the ascites version of the product.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Geminin antibody (ab104306)ab104306, at 3 µg/ml, staining Geminin in formalin-fixed paraffin-embedded Human lymph node by Immunohistochemistry.
This image was generated using the ascites version of the product.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Geminin antibody (ab104306)ab104306, at 1µg/ml, staining Geminin in formalin-fixed paraffin-embedded Human placenta by Immunohistochemistry.
This image was generated using the ascites version of the product.
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Immunocytochemistry/ Immunofluorescence - Anti-Geminin antibody (ab104306)ab104306, at 10 µg/ml, staining Geminin in HeLa cells by Immunofluorescence.
This image was generated using the ascites version of the product.
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Flow Cytometry - Anti-Geminin antibody (ab104306)Overlay histogram showing HeLa cells stained with ab104306 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab104306, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
This image was generated using the ascites version of the product.
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Immunocytochemistry/ Immunofluorescence - Anti-Geminin antibody (ab104306) This image is courtesy of an Abreview submitted by Melody JimenezImmunofluorescence analysis of Caki-2 (Clear cell renal carcinoma) cells, staining Geminin with ab104306.
Cells were fixed with formaldehyde, permeabilized with 0.25% Triton X-100 and blocked with 1% BSA for 30 minutes at 25oC. Primary antibody was concentrated using ab102778 Antibody Concentration Kit and conjugated to Rhodamine using ab102915 EasyLink Rhodamine Conjugation Kit. Samples were incubated with conjugated primary antibody (20 μg/ml in diluent) for 2 hours at 25oC.This image was generated using the ascites version of the product.
