Anti-Geminin antibody (ab264157)
Key features and details
- Rabbit polyclonal to Geminin
- Suitable for: IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Geminin antibody
See all Geminin primary antibodies -
Description
Rabbit polyclonal to Geminin -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanIP Human
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Immunogen
Synthetic peptide within Human Geminin aa 50-100. The exact sequence is proprietary. NP_056979.1
Database link: O75496 -
Positive control
- IHC-P: Human breast carcinoma. IP: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-Geminin antibody (ab264157)
Geminin was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264157 at 3 µg per reaction. Western blot was performed from the immunoprecipitate using a different Geminin antibody at 1 µg/ml.
Lane 1: ab264157 IP in HeLa whole cell lysate.
Lane 2: Control IgG.Exposure time: 3 mins.
Cells prepared using NETN lysis buffer.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Geminin antibody (ab264157)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast carcinoma tissue labeling Geminin with ab264157 at 1/5,000 dilution. Detection: DAB.
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
