Human PAR1/Thrombin Receptor ELISA Kit (ab277440)
Key features and details
- Sensitivity: 3 pg/ml
- Range: 2.87 pg/ml - 700 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human PAR1/Thrombin Receptor ELISA Kit
See all PAR1/Thrombin Receptor kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
3 pg/ml -
Range
2.87 pg/ml - 700 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 105.3 100% - 110% Plasma 113.2 103% - 125% Cell culture media 123 115% - 133% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human PAR1/Thrombin Receptor ELISA Kit (ab277440) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human PAR1/Thrombin Receptor in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human PAR1/Thrombin Receptor coated on a 96-well plate. Standards and samples are pipetted into the wells and Human PAR1/Thrombin Receptor present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human PAR1/Thrombin Receptor antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human PAR1/Thrombin Receptor bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml Biotinylated Anti-Human PAR1/Thrombin Receptor Antibody 2 vials Anti-Human PAR1 Thrombin Receptor coated Microplate 1 unit Human PAR1/Thrombin Receptor standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
High affinity receptor for activated thrombin coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelets activation and in vascular development. -
Tissue specificity
Platelets and vascular endothelial cells. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Post-translational
modificationsA proteolytic cleavage generates a new N-terminus that functions as a tethered ligand.
Phosphorylated; probably mediating desensitization prior to the uncoupling and internalization of the receptor. -
Cellular localization
Cell membrane. - Information by UniProt
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Alternative names
- CF2R
- Coagulation factor II (thrombin) receptor
- Coagulation factor II receptor
see all -
Database links
- Entrez Gene: 2149 Human
- Omim: 187930 Human
- SwissProt: P25116 Human
- Unigene: 482562 Human
Images
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Example data
These standard curves are for demonstration only. A standard curve must be run with each assay.
