Anti-SYT2 antibody (ab113545)
Key features and details
- Rabbit polyclonal to SYT2
- Suitable for: IHC-P, WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-SYT2 antibody
See all SYT2 primary antibodies -
Description
Rabbit polyclonal to SYT2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat, Dog, Human, Pig, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Common marmoset, Orangutan
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- This antibody gave a positive signal in the following Mouse tissue lysates: Brain; Spinal Cord; Cortex; Hippocampus; Cerebellum; Forebrain; Substantia Nigra. This antibody gave a positive result in IHC in the following FFPE tissue: Mouse normal brain.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab113545 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes IHC-P Use a concentration of 0.5 µg/ml. WB Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 47 kDa). Target
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Function
May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. -
Sequence similarities
Belongs to the synaptotagmin family.
Contains 2 C2 domains. -
Domain
The first C2 domain mediates Ca(2+)-dependent phospholipid binding.
The second C2 domain mediates interaction with Stonin 2. -
Cellular localization
Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cytoplasmic vesicle > secretory vesicle > chromaffin granule membrane. Synaptic vesicles and chromaffin granules. - Information by UniProt
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Database links
- Entrez Gene: 127833 Human
- Entrez Gene: 20980 Mouse
- Entrez Gene: 24805 Rat
- Omim: 600104 Human
- SwissProt: Q8N9I0 Human
- SwissProt: P46097 Mouse
- SwissProt: P29101 Rat
- Unigene: 25422 Human
see all -
Alternative names
- Synaptotagmin 2 antibody
- Synaptotagmin II antibody
- Synaptotagmin-2 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYT2 antibody (ab113545)This image is courtesy of an abreview submitted by Carl Hobbs (King's College London, United Kingdom)
IHC-P image of SYT2 staining on sections from rat thalamic nucleus (upper image) and the CA3 region of the rat hippocampus (lower image) using ab113545 (1:5000). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 mins at 21°C. ab113545 was diluted 1:5000 in TBS buffer (containing BSA and Azide) and sections were then incubated with ab113545 for 2 hours 10 minutes at 21°C. The secondary antibody used was Biotin conjugated Goat polyclonal to Rabbit IgG (1:250).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYT2 antibody (ab113545)This image is courtesy of an abreview submitted by Carl Hobbs (King's College London, United Kingdom)IHC-P image of SYT2 staining on sections from mouse sagittal hippocampus (upper image) and dentate nucleus of the mouse cerebellum (lower image) using ab113545 (1:5000). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 mins at 21°C. ab113545 was diluted 1:5000 in TBS buffer (containing BSA and Azide) and sections were then incubated with ab113545 for 2 hours at 21°C. The secondary antibody used was Biotin conjugated Goat polyclonal to Rabbit IgG (1:250).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYT2 antibody (ab113545)This image is courtesy of an abreview submitted by Carl Hobbs (King's College London, United Kingdom)IHC-P image of SYT2 staining on Marmoset cerebellum sections using ab113545(1:7000). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 mins at 21°C. ab113545 was diluted 1:7000 in TBS buffer (containing BSA and Azide) and sections were then incubated with ab113545 for 2 hours at 21°C. The secondary antibody used was Biotin conjugated Goat polyclonal to Rabbit IgG (1:250).
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Western blot - Anti-SYT2 antibody (ab113545)All lanes : Anti-SYT2 antibody (ab113545) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Spinal Cord (Mouse) Tissue Lysate
Lane 3 : Mouse Cortex Tissue Lysate
Lane 4 : Mouse Hippocampus Tissue Lysate
Lane 5 : Cerebellum Mouse Tissue Lysate
Lane 6 : Forebrain (Mouse) Tissue Lysate
Lane 7 : Mouse Substantia Nigra Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes
The predicted molecular weight of SYT2 is 47 kDa (SwissProt), however we expect to observe a banding pattern around 70 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYT2 antibody (ab113545)IHC image of SYT2 staining in Mouse normal brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab113545, 0.5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
References (0)
ab113545 has not yet been referenced specifically in any publications.
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYT2 antibody (ab113545) This image is courtesy of an abreview submitted by Carl Hobbs (King's College London, United Kingdom)
IHC-P image of SYT2 staining on sections from rat thalamic nucleus (upper image) and the CA3 region of the rat hippocampus (lower image) using ab113545 (1:5000). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 mins at 21°C. ab113545 was diluted 1:5000 in TBS buffer (containing BSA and Azide) and sections were then incubated with ab113545 for 2 hours 10 minutes at 21°C. The secondary antibody used was Biotin conjugated Goat polyclonal to Rabbit IgG (1:250).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYT2 antibody (ab113545) This image is courtesy of an abreview submitted by Carl Hobbs (King's College London, United Kingdom)
IHC-P image of SYT2 staining on sections from mouse sagittal hippocampus (upper image) and dentate nucleus of the mouse cerebellum (lower image) using ab113545 (1:5000). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 mins at 21°C. ab113545 was diluted 1:5000 in TBS buffer (containing BSA and Azide) and sections were then incubated with ab113545 for 2 hours at 21°C. The secondary antibody used was Biotin conjugated Goat polyclonal to Rabbit IgG (1:250).
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYT2 antibody (ab113545) This image is courtesy of an abreview submitted by Carl Hobbs (King's College London, United Kingdom)
IHC-P image of SYT2 staining on Marmoset cerebellum sections using ab113545(1:7000). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 mins at 21°C. ab113545 was diluted 1:7000 in TBS buffer (containing BSA and Azide) and sections were then incubated with ab113545 for 2 hours at 21°C. The secondary antibody used was Biotin conjugated Goat polyclonal to Rabbit IgG (1:250).
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Western blot - Anti-SYT2 antibody (ab113545)All lanes : Anti-SYT2 antibody (ab113545) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Spinal Cord (Mouse) Tissue Lysate
Lane 3 : Mouse Cortex Tissue Lysate
Lane 4 : Mouse Hippocampus Tissue Lysate
Lane 5 : Cerebellum Mouse Tissue Lysate
Lane 6 : Forebrain (Mouse) Tissue Lysate
Lane 7 : Mouse Substantia Nigra Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes
The predicted molecular weight of SYT2 is 47 kDa (SwissProt), however we expect to observe a banding pattern around 70 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYT2 antibody (ab113545)
IHC image of SYT2 staining in Mouse normal brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab113545, 0.5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
